Method and kit of detecting the absence of micro-organisms

ABSTRACT

Methods of detecting the absence or presence of a micro-organism in a sample comprising: contacting the sample with a nucleic acid molecule which acts as a substrate for nucleic acid modifying activity of the micro-organism in the sample, incubating the thus contacted sample under conditions suitable for nucleic acid modifying activity; and specifically determining the absence or presence of a modified nucleic acid molecule resulting from the action of the nucleic acid modifying activity on the substrate nucleic acid molecule to indicate the absence or presence of the micro-organism. Corresponding kits are also provided.

CROSS-REFERENCE TO RELATED APPLICATIONS

The present application is the U.S. national stage of InternationalApplication PCT/GB2015/052006, filed on Jul. 10, 2015, whichinternational application was published on Jan. 14, 2016 asInternational Publication No. WO2016/005768. The InternationalApplication claims priority to British Patent Application No. GB1412316.0, filed on Jul. 10, 2014, the contents of which areincorporated herein by reference in their entireties.

FIELD OF THE INVENTION

The present invention relates generally to the field of detecting theabsence or presence of microorganisms in a sample. The methods typicallyrely upon measuring microbial enzyme activity (if any) present in asample and may relate to such methods which are capable of beingperformed using nucleic acid amplification techniques such as thepolymerase chain reaction. The methods of the invention therefore enabledetermination of the absence and presence of microbial pathogens insamples such as un-purified blood, blood culture and other body fluids.This invention also relates to reagents for use in such methods, and totest kits comprising such reagents useful for carrying out the methods.

BACKGROUND TO THE INVENTION

Measuring the presence and levels of certain molecules which areassociated with cell viability is important in a number of contexts. Forexample, measuring levels of ATP is useful in mammalian cells for growthanalysis and toxicology purposes. Culture approaches can be used todetect small numbers of bacteria but such techniques require severaldays to complete, especially when attempting to detect small numbers ofbacteria and also when detecting slower growing microorganisms.

Detection of adenylate kinase as an indicator of viability has also beenproposed (Squirrell D J, Murphy M J, Leslie R L, Green J C D: Acomparison of ATP and adenylate kinase as bacterial cell markers:correlation with agar plate counts. WO96/002665 describes a method fordetermining the presence and/or amount of microorganisms and/or theirintracellular material present in a sample characterized in that theamount of adenylate kinase in the sample is estimated by mixing it withadenosine diphosphate (ADP), determining the amount of adenosinetriphosphate (ATP) produced by the sample from this ADP, and relatingthe amount of ATP so produced to the presence/or amount of adenylatekinase and to microorganisms and/or their intracellular material,wherein the conversion of ADP to ATP is carried out in the presence ofmagnesium ions at a molar concentration sufficient to allow maximalconversion of ADP to ATP.

In WO2009/007719, ligases, in particular NAD− dependent ligases, aredisclosed as a useful indicator of the presence of a (viable)microorganism in a sample. Ligases are enzymes which catalyze ligationof nucleic acid molecules. The ligation reaction requires either ATP orNAD+ as co-factor depending upon the ligase concerned. In thisdisclosure, the use of NAD− dependent ligase activity is utilized as anindicator of the presence of a (viable) microorganism in a sample.

WO2011/130584 describes a method for detection of viable microorganismsbased on detection of DNA or RNA polymerases in which a sample iscontacted with a nucleic acid substrate that acts as a substrate formicrobial polymerase, incubated under conditions suitable for polymeraseactivity from intact microorganisms and any resulting nucleic acidproduct is determined using a nucleic acid amplification technique suchas quantitative polymerase chain reaction. Such assays have been termed“ETGA assays”, where ETGA stands for Enzymatic Template Generation andAmplification. A problem with ETGA assays for viable microorganisms incrude samples is the presence of contaminating polymerase activityoutside the microorganisms arising from host (e.g. human) cells and deadmicroorganisms. The ETGA assay is unable to distinguish microorganismpolymerase activity from that of the host or from dead microorganisms.

Applicant's co-pending application WO2010/119270 describes a method forremoving enzyme activity (in this case, DNA ligase) outside intactmicroorganisms and this can be used also for removal of contaminationnucleic acid polymerase activity.

DESCRIPTION OF THE INVENTION

However, the conditions used in WO2010/119270 for removing contaminatingactivity include incubation at high pH (around pH 11) for 20 min. Whilstuseful, these conditions have been found by the inventors to bedetrimental to particular bacterial strains such as certain clinicalstrains of H. influenzae.

It has further been discovered that treatment with high pH does not, inall cases, remove all the extramicrobial nuclease activity in samplessuch as blood culture and that this nuclease activity can have adetrimental effect on the assay by degrading the nucleic acid substrateused in the test. Nucleic acid amplification assays may include aninternal control probe to monitor for the correct functioning of theamplification reaction (see for example WO2013/103744 where this isapplied to a DNA polymerase assay similar to that of WO2011/130584).However, this internal control is added as part of the nucleic acidamplification reagent mix and would not detect prior nuclease activity.An additional problem with the method as described in WO2011/130584 is arelative lack of sensitivity in detecting yeast such as C. albicans andC. glabrata.

In rapid testing, the art has focused on detection of the presence ofmicroorganisms rather than determining their absence. By “determiningtheir absence” the applicants do not mean that the sample is necessarilysterile but may have an organism load that is sufficiently low as to benegative for practical purposes. For example, blood cultures are oftentaken from patients suspected of having bloodstream infections which canbe associated with sepsis, a condition that can be rapidly fatal if leftuntreated. It is routine for clinical microbiology laboratories toincubate such specimens for at least five days before reporting anegative result, during which time the patients are often kept on broadspectrum antibiotics. Typically up to 90% of such patients are negative,and so a large number of patients are left for 5 days on antibiotictherapy that is not necessary for their condition. A faster method fordetermining a negative result (relative to a five-day blood culture)would be of significant value in reducing the cost of unnecessaryantibiotic therapy and provide health benefits in terms of reducing therisks of C. difficile infection, antibiotic toxicity and lowering therate of increase in antimicrobial resistance.

The inventors have devised and tested a range of improvements toexisting ETGA assays with a view to optimising determination of theabsence or presence of a micro-organism in a sample. The foundation ofthe invention is thus methods of detecting the absence or presence of amicro-organism in a sample comprising:

-   -   (a) contacting the sample with a nucleic acid molecule which        acts as a substrate for nucleic acid modifying activity of the        micro-organism in the sample,    -   (b) incubating the thus contacted sample under conditions        suitable for nucleic acid modifying activity; and    -   (c) specifically determining the absence or presence of a        modified nucleic acid molecule resulting from the action of the        nucleic acid modifying activity on the substrate nucleic acid        molecule to indicate the absence or presence of the        micro-organism. Various developments of this basic assay format        are presented herein.

Accordingly, in a first aspect, the invention provides a method ofdetecting the absence or presence of a micro-organism in a samplecomprising:

-   -   (a) contacting the sample with a nucleic acid molecule which        acts as a substrate for nucleic acid modifying activity of the        micro-organism in the sample,    -   (b) incubating the thus contacted sample under conditions        suitable for nucleic acid modifying activity; and    -   (c) specifically determining the absence or presence of a        modified nucleic acid molecule resulting from the action of the        nucleic acid modifying activity on the substrate nucleic acid        molecule to indicate the absence or presence of the        micro-organism, characterised in that the nucleic acid molecule        is modified so as to protect it from nuclease activity.

In the context of the present invention, the nucleic acid molecule ispre-modified so as to protect it from nuclease activity i.e. the nucleicacid molecule is modified so as to protect it from nuclease activitybefore it is contacted with the sample in step (a).

The inventors have determined that protection of the substrate nucleicacid molecule from nuclease activity is advantageous in the context ofthe assays of the invention. More specifically as shown herein,incorporation of protected nucleic acid molecules into the methods ofthe invention improves sensitivity of detection. Any suitable means maybe employed in order to protect the nucleic acid molecule from nucleaseactivity. Non-limiting examples include incorporation of methylationinto the nucleic acid molecule, end modification such as protection ofthe 3′ and/or 5′ ends and incorporation of synthetic nucleotides. Inspecific embodiments, the synthetic nucleotides comprisephosphorothioate nucleotides and/or locked nucleic acid nucleotides.Preferably, the synthetic nucleotides are phosphorothioate nucleotides.In certain embodiments, the synthetic nucleotides replace at least oneup to all of the nucleotides in the nucleic acid molecule.

The inventors have further determined that, compared to prior ETGAassays, increasing the concentration of the nucleic acid molecule in thereaction can lead to improved results. Thus, in a further aspect theinvention provides a method of detecting the absence or presence of amicro-organism in a sample comprising:

-   -   (a) contacting the sample with a nucleic acid molecule which        acts as a substrate for nucleic acid modifying activity of the        micro-organism in the sample,    -   (b) incubating the thus contacted sample under conditions        suitable for nucleic acid modifying activity; and    -   (c) specifically determining the absence or presence of a        modified nucleic acid molecule resulting from the action of the        nucleic acid modifying activity on the substrate nucleic acid        molecule to indicate the absence or presence of the        micro-organism, characterised in that the nucleic acid molecule        is added to the sample at a concentration of at least 2 nM but        less than 50 nM.

Previously, in such assays, the nucleic acid substrate has been utilisedat a concentration of 1 nM. The inventors have determined thatincreasing this concentration to less than 50 nM, such as 2 nM, 5 nM,7.5 nM or 10 nM, results in improved sensitivity of detection. At orabove 50 nM, the improved sensitivity is lost due to the increase infalse positives resulting from the assay. The concentration as statedherein, is typically the concentration in the lysis mixture used to lysethe micro-organisms if present in the sample. Thus, step (a) ofcontacting the sample with the substrate nucleic acid molecule typicallyinvolves addition of the substrate in a lysis mixture which lyses themicro-organisms if present in the sample. Further details of the lysisreagent/mixture are provided herein.

The inventors have still further determined that increasing theconcentration of free nucleotides in the reaction further serves toimprove assay sensitivity. Accordingly, in some embodiments, the methodsof the invention comprise adding to the sample deoxyribonucleotidetriphosphates (dNTPs) at a concentration of more than 50 μM, such as 55to 300 μM, or 60 to 250 μM, or 75 to 200 μM in particular at least 100μM. The dNTPs may be added in either step (a) and/or step (b) in someembodiments. The concentration as stated herein, is typically theconcentration in the lysis mixture used to lyse the micro-organisms ifpresent in the sample. Thus, step (a) of contacting the sample with thesubstrate nucleic acid molecule typically involves addition of thesubstrate in a lysis mixture which lyses the micro-organisms if presentin the sample, in which the lysis mixture contains the dNTPs. Furtherdetails of the lysis reagent/mixture are provided herein.

As discussed above, the inventors have discovered that while the high pHconditions adopted in WO2010/119270 for removing contaminating activityare useful, these conditions may be detrimental to particular bacterialstrains such as certain clinical strains of H. influenzae. Accordingly,in a further aspect the invention provides a method of detecting theabsence or presence of a micro-organism in a sample, the samplecontaining a non-microorganism source of nucleic acid modifying activitycomprising:

-   -   (a) treating the sample under high pH conditions for no more        than 8 minutes in order to inhibit the non-microorganism source        of nucleic acid modifying activity (whilst not affecting the        nucleic acid modifying activity of the microorganism in the        sample),    -   (b) contacting the sample with a nucleic acid molecule which        acts as a substrate for nucleic acid modifying activity of the        micro-organism in the sample,    -   (c) incubating the thus contacted sample under conditions        suitable for nucleic acid modifying activity; and    -   (d) specifically determining the absence or presence of a        modified nucleic acid molecule resulting from the action of the        nucleic acid modifying activity on the substrate nucleic acid        molecule to indicate the absence or presence of the        microorganism.

The duration of the high pH conditions is less than 20 minutes and maybe not more than 10, 9, 8, 7, 6 or 5 minutes and may be around 5, 6, 7,8, 9 or 10 minutes. In yet further embodiments, the treatment is carriedout for between around 2 and 15 minutes, such as around 5 minutes. By“around” is meant plus or minus 30 seconds.

Any suitable reagent may be added to the sample in order to provide highpH conditions. In particular embodiments, the high pH conditionscomprise contacting the sample with an alkali. In particularembodiments, NaOH or Na2CO3 is used. In specific embodiments, theconcentration of the NaOH or Na2CO3 is around 5 mM or greater.

The high pH conditions typically inhibit the activity of nucleic acidmodifying enzymes including ATP-dependent ligase and polymerases fromnon-microorganism sources such as mammalian cells, but do not inhibitthe activity of the microbial ligases or polymerases. This may be due tothe greater resistance of microbial enzymes to these conditions and/orto differential lysis conditions employed in the methods to ensure thatonly the non-microorganism enzymes are exposed to the high pHconditions. High pH is generally a pH of at least around 10, such asaround 10, 11, 12, 13 or 14. Low pH is generally a pH of less than orequal to around 4, such as around 4, 3, 2, or 1. By “around” is meant0.5 of a pH unit either side of the stated value. Altering the pH of thesample may be achieved using any suitable means, as would be readilyappreciated by one skilled in the art. Microbial enzymes such aspolymerases and ligases may be resistant to extremes of pH, whereasmammalian ligases may be inactivated under the same pH conditions. Thispermits selective detection of microbial ligases in a sample containingboth mammalian cells and microbial cells. In specific embodiments, theconditions that inhibit the activity of non-microorganism nucleic acidmodifying activity, such as ATP-dependent ligase, from mammalian cellsbut which do not inhibit the activity of the microorganism source ofnucleic acid modifying activity, such as microbial ligases, comprisetreating the sample with sodium hydroxide (NaOH) or sodium carbonate(Na2CO3). Such agents can readily be used, as shown herein, to increasethe pH of the sample to high pH thus inactivating mammalian ligaseactivity whilst leaving the microbial (fungal and bacterial) ligasesactive. Suitable concentrations and volumes of the appropriate agent canbe applied by a skilled person. In certain embodiments, however, theNaOH is at least around 5 mM NaOH. In some embodiments, the alkaliconcentration is no more than 10 mM, such as 5, 6, 7, 8, 9 or 10 mM.

In further embodiments, the pH is around 12 to inactivate mammaliannucleic acid modifying activity (such as polymerase and/or ATP-dependentligase activity), but not microbial nucleic acid modifying activity(such as polymerase and/or ligase activity). In specific embodiments, pHconditions may be increased to at least around 11, or at least 11.2.This treatment may result in lysis of micro-organisms in the sample andthus lead to nucleic acid modifying activity (e.g. polymerase and/orligase) release into the sample. This permits detection of nucleic acidmodifying activity (e.g. polymerases and/or ligases) in the sample,originating from the micro-organism, without the need for a separatecell lysis step. Under these conditions, mammalian ligases (such asblood ATP-dependent ligases) are inactivated. However, typically themethods include a separate step for lysing microorganisms in the sample,as discussed in greater detail herein below.

In some embodiments, the treatment under high pH conditions is stoppedby adding a reagent to lower the pH. Suitable reagents include a bufferand/or an acid. In specific embodiments, the buffer comprises a Tris-HClbuffer (e.g. pH 7.2 or 8). Other suitable agents for lowering the pHinclude acids such as hydrochloric acid (HCl) and sulphuric acid(H2SO4). These (and other) acids may be incorporated into a buffer aswould be readily appreciated by one skilled in the art. These steps maybe incorporated into step (a) of the method outlined above.

In specific embodiments, step (a) is performed at a temperature betweenaround (to mean plus or minus 0.5 degrees) 15 and 30 degrees Celsius. Incertain embodiments, step (a) is performed at room temperature. Theentirety of the methods described herein may be performed at thesetemperatures.

In a more specific recitation of these methods, the invention furtherprovides a method of detecting the absence or presence of amicro-organism in a sample, the sample containing a non-micro-organismsource of nucleic acid modifying activity comprising:

-   -   (i) incubation of the sample with a reagent that lyses        non-microorganisms if present in the sample but does not lyse        microorganisms in the sample    -   (ii) optionally separation of the lysed cell material from the        intact microorganisms (if any) in the sample    -   (iii) contacting the (separated) intact microorganisms (if any)        in the sample with a high pH reagent and incubating for no more        than 5 minutes in order to inhibit the non-microorganism source        of nucleic acid modifying activity (whilst not affecting the        nucleic acid modifying activity of the microorganism in the        sample)    -   (iv) adding a pH lowering reagent in order to stop the        incubation at high pH    -   (v) separation of the microorganisms if present in the sample        from the pH modifying reagents    -   (vi) lysis of any separated microorganisms    -   (vi) contacting the sample with a nucleic acid molecule which        acts as a substrate for nucleic acid modifying activity of the        micro-organism in the sample,    -   (vii) incubating the thus contacted sample under conditions        suitable for nucleic acid modifying activity; and    -   (viii) specifically determining the absence or presence of a        modified nucleic acid molecule resulting from the action of the        nucleic acid modifying activity on the substrate nucleic acid        molecule to indicate the absence or presence of the        micro-organism.

Step (ii) is an optional step because, in some embodiments, the lysedcell material does not need to be separated from the intactmicroorganisms. This is because step (iii) is used to inhibit thenucleic acid modifying activity found in the lysed cell material in anycase.

By “lysed cell material” is meant the product of lysis of thenon-microorganisms. This includes the cell membranes and intracellularcontent of the lysed cells.

More specifically, the methods may comprise the steps of:

-   -   (i) incubation of the sample with a reagent that lyses        non-micro-organisms if present in the sample but does not lyse        micro-organisms in the sample    -   (ii) centrifugation of the sample to form a pellet containing        micro-organisms if present in the sample    -   (iii) removal of the supernatant from the pellet    -   (iv) re-suspending the pellet in a high pH reagent and        incubating for no more than 8 minutes in order to inhibit the        non-micro-organism source of nucleic acid modifying activity        (whilst not affecting the nucleic acid modifying activity of the        micro-organism in the sample)    -   (v) adding a pH lowering reagent in order to stop the incubation        at high pH    -   (vi) a second centrifugation of the sample to form a pellet        containing micro-organisms if present in the sample    -   (vi) removal of the supernatant from the pellet    -   (vii) lysing any micro-organisms in the pellet    -   (viii) contacting the sample with a nucleic acid molecule which        acts as a substrate for nucleic acid modifying activity of the        micro-organism in the sample,    -   (ix) incubating the thus contacted sample under conditions        suitable for nucleic acid modifying activity; and    -   (x) specifically determining the absence or presence of a        modified nucleic acid molecule resulting from the action of the        nucleic acid modifying activity on the substrate nucleic acid        molecule to indicate the absence or presence of the        micro-organism.

In specific embodiments, step (iii) or (iv) respectively, or the entiremethod is performed at a temperature between 15 and 30 degrees Celsius.Alternatively, step (iii) or (iv) respectively or the entire method maybe performed at room temperature.

The reagent that lyses non-micro-organisms, in particular mammaliancells, if present in the sample but does not lyse micro-organisms in thesample may be any suitable reagent. The reagent may include a surfactantor detergent in some embodiments, such as a non-ionic detergent.Suitable examples include polyethylene glycol sorbitan monolaurate(Tween 20), for example at 5% w/v. The reagent may include a saponin,for example at 5% w/v. The reagent may include a metal halide salt, suchas sodium chloride, for example at 8.5 g/l. The reagent may include amixture of all three components. The sample may be mixed with thereagent under suitable conditions to ensure lysis ofnon-micro-organisms, in particular mammalian cells, if present in thesample but no (or insignificant) lysis of micro-organisms if present inthe sample. The sample may be exposed to the reagent for a period ofbetween around 5 and 30 minutes, such as 5, 10, 15, 20, 25 or 30minutes. This step may be performed at any suitable temperature, forexample between 15 and 30 degrees Celsius or at room temperature.

Where used, separation of the lysed cell material from the intactmicroorganisms (if any) in the sample may be performed by any suitablemethod. It may for example rely upon a form of affinity purification,such as an (polyclonal) antibody-based approach. It may rely uponfiltration in some embodiments. Separation may rely upon centrifugationof the sample to form a pellet containing micro-organisms if present inthe sample. Centrifugation of the sample may be performed at anysuitable speed and for any suitable duration. For example, the samplemay be centrifuged at a speed of between 3000 and 10000 g, such asaround 7000 g or 7300 g. The sample may be centrifuged for a suitableperiod of time to ensure successful lysis of non-micro-organisms, inparticular mammalian cells, if present in the sample but no orinsignificant lysis of micro-organisms in the sample. This may bedetermined in conjunction with the speed of centrifugation. The timeperiod may be between around 1 and 30 minutes, such as 1, 2, 3, 4, 5,10, 15, 20, 25 or 30 minutes. This step may be performed at any suitabletemperature, for example between 15 and 30 degrees Celsius or at roomtemperature. Following separation, the lysed cell material may bediscarded (in the form of a supernatant) and the non-lysed cellsretained (for example as a pellet).

The discussion provided above in respect of the more general methodsapplies mutatis mutandis here. Thus, in some embodiments the high pHreagent comprises NaOH or Na2CO3. In some embodiments, the concentrationof the high pH reagent is around 5 mM or greater. In certainembodiments, the pH lowering reagent comprises a buffer or an acid, suchas a Tris-HCl buffer. The buffer may be a pH 7.2 or 8 buffer in specificembodiments.

Following exposure to the pH modifying agent, any microorganisms in thesample are separated from the pH modifying conditions. This may beachieved by a second centrifugation of the sample to form a pelletcontaining micro-organisms if present in the sample, followed by removalof the supernatant from the pellet. Suitable centrifugation conditionsare discussed above.

The method then requires lysis of any separated microorganisms to permitdetection of nucleic acid modifying activity. This may be achieved byaddition of a lysis mixture. The lysis mixture is generally useful inthe methods of the invention. The lysis mixture may include a specificmixture of components to ensure efficient lysis of microorganismswithout adversely affecting nucleic acid modifying activity within thecells. The components may be selected from carrier/serum proteins suchas BSA, surfactants/detergents, metal halide salts, buffers, chelatorsetc. In its basic form, the lysis mixture of the invention may includethe following components:

-   -   1. A surfactant/detergent    -   2. Serum protein such as albumin (e.g. BSA)    -   3. Buffer    -   4. Nucleotides, such as dNTPs    -   5. Nucleic acid molecule (acting as a substrate in the assays of        the invention).

A suitable lysis mixture is set forth below in table 1 and forms aseparate aspect of the invention:

TABLE 1 Lysis mixture components L1 Bovine serum albumin 1.5% w/v TritonX100 1.5% v/v Tween 20 1.5% v/v L2 Ammonium sulphate 2.64 g/L Magnesiumsulphate heptahydrate 0.98 g/L Potassium chloride 1.5 g/L Tris-HCl, pH8.0 40 mM dNTP (A, G, C, T) 500 μM L3 ETGA substrate (nucleic acid 0.001μM-0.01 μM molecule) Tris-HCl, pH 8.5 20 mM KCl 10 mM EDTA 10 μM

Exemplary concentrations of each component are listed but may bemodified as would be readily appreciated by one skilled in the art.

Lysis may also require disruption of the cells. For example, the cellsmay be disrupted using the lysis mixture in combination with physicaland/or enzymatic means. In some embodiments, physical disruption employsa disruptor. The disruptor may incorporate beads such as glass beads tolyse the cells. Suitable apparatus are commercially available andinclude the Disruptor Genie manufactured by Scientific Industries, Inc.Enzymatic disruption may require use of an agent selected fromlysostaphin, lysozyme and/or lyticase in some embodiments.

As indicated in table 1, the step of contacting the sample with anucleic acid molecule which acts as a substrate for nucleic acidmodifying activity of the micro-organism in the sample may includeadding the nucleic acid molecule to the lysis mixture.

The sample is then incubated under conditions suitable for nucleic acidmodifying activity. This may involve incubation at an optimumtemperature for nucleic acid modifying activity. For example, the samplemay be incubated at a temperature between around 15 and 40 degreesCelsius, such as around 37 degrees Celsius. This may be for any suitableperiod of time, for example between 5 and 60 minutes, such as around 5,10, 15, 20, 25 or 30 minutes. Following this, the nucleic acid modifyingactivity may be inactivated prior to the modified nucleic acid moleculedetection step. This may be achieved by elevating the temperature, forexample to a temperature above 60 degrees Celsius, such as 95 degreesCelsius for a suitable time period. This may be a relatively short timeperiod such as 1, 2, 3, 4, 5, 10, 15 or more minutes.

Specifically determining the absence or presence of a modified nucleicacid molecule resulting from the action of the nucleic acid modifyingactivity on the substrate nucleic acid molecule to indicate the absenceor presence of the micro-organism may be performed by any suitablemethod as discussed herein. Preferred methods are nucleic acidamplification based and may permit quantification of the nucleic acidmodifying activity (and thus microorganisms) in the sample.

The inventors have also investigated the use of an internal positivecontrol (IPC) molecule in the context of the ETGA methods. Inparticular, the invention may rely upon inclusion of the IPC with thesubstrate nucleic acid molecule so that the IPC is exposed to identicalconditions. They have found that residual nuclease activity in thesample may affect the substrate added to the sample (particularly in thelysis mixture, as defined herein). Thus, there is an advantage inprotecting the IPC from nuclease activity. Accordingly, the inventionalso provides a method of detecting the absence or presence of amicro-organism in a (liquid) sample, the sample potentially containing anon-micro-organism source of nuclease activity comprising:

-   -   (i) incubation of the sample with a reagent that lyses        non-microorganisms if present in the sample but does not lyse        microorganisms in the sample    -   (ii) separation of the lysed cell material from the intact        microorganisms (if any) in the sample and/or inactivation of the        lysed cell material    -   (iii) lysing any microorganisms following the separation and/or        inactivation    -   (iv) contacting the sample with a nucleic acid molecule which        acts as a substrate for nucleic acid modifying activity of the        micro-organism in the sample together with an internal positive        control (IPC) nucleic acid molecule,    -   (v) incubating the thus contacted sample under conditions        suitable for nucleic acid modifying activity; and    -   (vi) specifically determining the absence or presence of a        modified nucleic acid molecule resulting from the action of the        nucleic acid modifying activity on the substrate nucleic acid        molecule to indicate the absence or presence of the        micro-organism, characterised in that the IPC nucleic acid        molecule is modified so as to protect it from nuclease activity.

Separation in step (ii) is an optional step because, in someembodiments, the lysed cell material does not need to be separated fromthe intact microorganisms. In some embodiments, an alternative oradditional step of inactivating the nucleic acid modifying activityfound in the lysed cell material is performed. Any suitable inactivationtechnique may be employed as discussed herein. For example, inactivationmay be of nucleic acid modifying activity and/or nuclease activity inthe lysed cell material. Inactivation may be achieved using any suitablemeans, for example high pH treatment as discussed herein. The fact thatthe microorganisms remain intact may protect them from an inactivationtreatment.

Similarly, the invention also provides a method of detecting the absenceor presence of a micro-organism in a (liquid) sample, the samplepotentially containing a non-micro-organism source of nuclease activitycomprising:

-   -   (a) centrifugation of the sample to form a pellet containing        micro-organisms if present in the sample    -   (b) removal of the supernatant from the pellet    -   (c) lysing any micro-organisms in the pellet    -   (d) contacting the sample with a nucleic acid molecule which        acts as a substrate for nucleic acid modifying activity of the        micro-organism in the sample together with an internal positive        control (IPC) nucleic acid molecule,    -   (e) incubating the thus contacted sample under conditions        suitable for nucleic acid modifying activity; and    -   (f) specifically determining the absence or presence of a        modified nucleic acid molecule resulting from the action of the        nucleic acid modifying activity on the substrate nucleic acid        molecule to indicate the absence or presence of the        micro-organism, characterised in that the IPC nucleic acid        molecule is modified so as to protect it from nuclease activity.

The protected IPC is particularly advantageous in the context of use ofprotected substrate molecules. Accordingly, in some embodiments, the(substrate) nucleic acid molecule is also modified so as to protect itfrom nuclease activity. This ensures that both nucleic acid moleculesare protected and subjected to the same conditions. Any suitable meansmay be employed in order to protect the nucleic acid molecules fromnuclease activity. Non-limiting examples include incorporation ofmethylation into the nucleic acid molecules, end modification such asprotection of the 3′ and/or 5′ ends and incorporation of syntheticnucleotides. In specific embodiments, the synthetic nucleotides comprisephosphorothioate nucleotides and/or locked nucleic acid nucleotides.Preferably, the synthetic nucleotides are phosphorothioate nucleotides.In certain embodiments, the synthetic nucleotides replace at least oneup to all of the nucleotides in the nucleic acid molecules. In specificembodiments, the IPC and substrate nucleic acid molecule are modified inthe same manner. This is with a view to providing as equal as possibleprotection from nuclease activity.

In the context of the present invention, if the IPC is modified so as toprotect it from nuclease activity, the IPC is pre-modified so as toprotect it from nuclease activity i.e. the IPC is modified so as toprotect it from nuclease activity before it is contacted with thesample.

The invention also contemplates using the IPC in order to monitorpotential contaminating nuclease activity in the sample. Accordingly,the invention also provides a method of detecting the absence orpresence of a micro-organism in a (liquid) sample, the samplepotentially containing a non-micro-organism source of nuclease activitycomprising:

-   -   (i) incubation of the sample with a reagent that lyses        non-micro-organisms if present in the sample but does not lyse        micro-organisms in the sample    -   (ii) separation of the lysed cell material from the intact        microorganisms (if any) in the sample and/or inactivation of the        lysed cell material    -   (iii) lysing any microorganisms following the separation and/or        inactivation    -   (iv) contacting the sample with a nucleic acid molecule which        acts as a substrate for nucleic acid modifying activity of the        micro-organism in the sample together with an internal positive        control (IPC) nucleic acid molecule,    -   (v) incubating the thus contacted sample under conditions        suitable for nucleic acid modifying activity; and    -   (vi) specifically determining the absence or presence of a        modified nucleic acid molecule resulting from the action of the        nucleic acid modifying activity on the substrate nucleic acid        molecule to indicate the absence or presence of the        micro-organism, characterised in that the IPC nucleic acid        molecule is susceptible to nuclease activity and is used to        identify contaminating nuclease activity in the pellet.

Separation in step (ii) is an optional step because, in someembodiments, the lysed cell material does not need to be separated fromthe intact microorganisms. In some embodiments, an alternative oradditional step of inactivating the nucleic acid modifying activityfound in the lysed cell material is performed. Any suitable inactivationtechnique may be employed as discussed herein. For example, inactivationmay be of nucleic acid modifying activity and/or nuclease activity inthe lysed cell material. Inactivation may be achieved using any suitablemeans, for example high pH treatment as discussed herein. The fact thatthe microorganisms remain intact may protect them from an inactivationtreatment.

Similarly, there is further provided a method of detecting the absenceor presence of a micro-organism in a (liquid) sample, the samplepotentially containing a non-micro-organism source of nuclease activitycomprising:

-   -   (a) centrifugation of the sample to form a pellet containing        micro-organisms if present in the sample    -   (b) removal of the supernatant from the pellet    -   (c) lysing any micro-organisms in the pellet    -   (d) contacting the sample with a nucleic acid molecule which        acts as a substrate for nucleic acid modifying activity of the        micro-organism in the sample together with an internal positive        control (IPC) nucleic acid molecule,    -   (e) incubating the thus contacted sample under conditions        suitable for nucleic acid modifying activity; and    -   (f) specifically determining the absence or presence of a        modified nucleic acid molecule resulting from the action of the        nucleic acid modifying activity on the substrate nucleic acid        molecule to indicate the absence or presence of the        micro-organism, characterised in that the IPC nucleic acid        molecule is susceptible to nuclease activity and is used to        identify contaminating nuclease activity in the pellet.

Typically, steps (iii) and (iv) or (c) and (d) are performed together.

In specific embodiments, the (substrate) nucleic acid molecule used inthe methods of the invention is at least partially double stranded andcomprises uracil residues in the complementary strand and the step ofspecifically determining the absence or presence of the modified nucleicacid molecule comprises adding Uracil DNA Glycosylase (UDG) to thesample in order to degrade the uracil residues in the complementarystrand.

In certain embodiments, the first strand of the partially doublestranded (substrate) nucleic acid molecule comprises (or consists of)synthetic nucleotides (e.g. phosphorothioate nucleotides) and the second(complementary) strand comprises (or consists of) uracil residues and,optionally, synthetic nucleotides (e.g. phosphorothioate nucleotides).Preferably, the double stranded region encompasses the 3′ end regions ofthe first and second (complementary) strands. Preferably, the doublestranded region is at least 5, at least 10, at least 15, at least 20 orat least 25 nucleotides; optionally, the double stranded region is nomore than 50 nucleotides. The first strand may be extended during anincubation step, as described herein, using unprotected (or standard)dNTPs by the polymerase activity of a micro-organism in the sample toform an extended first strand that comprises unprotected (or standard)nucleotides. This step relies upon using the second strand as template(upstream of the region of complementarity between the first and secondstrands). Following the incubation step, the second (complementary)strand may be degraded by adding Uracil DNA Glycosylase (UDG) to thesample leaving the extended first strand as a single stranded moleculecomprising synthetic nucleotides and unprotected nucleotides. Followingdegradation of the second strand, the extended first strand of the(substrate) nucleic acid molecule may be detected in an amplificationstep. The inventors have found that the use of a partially doublestranded (substrate) nucleic acid molecule as described above improvesthe detection of a micro-organism in the sample.

In certain embodiments, the IPC nucleic acid molecule comprisesidentical primer binding sites to the nucleic acid molecule such thatthere is competition for primer binding (in step (vi) or (f) of themethod).

In further embodiments, a nucleic acid probe is added (in step (vi) or(f)) which binds to a target probe sequence within the nucleic acidmolecule. Typically, the probe binds to the sense strand of the nucleicacid molecule. In other embodiments, a further nucleic acid probe isadded in step (vi) or (f) respectively which binds to a target probesequence within the IPC nucleic acid molecule. In specific embodiments,the nucleic acid probe does not bind to the IPC nucleic acid moleculeand the further nucleic acid probe does not bind to the nucleic acidmolecule. The nucleic acid probe and/or further nucleic acid probe maybe labelled. Preferably, they are differently labelled.

In specific embodiments, the complementary strand of the nucleic acidmolecule comprises a modification at the 3′ end to prevent extension.This modification may comprise incorporation of a non-extendiblenucleotide. In specific embodiments, the non-extendible nucleotide is adideoxy nucleotide triphosphate (ddNTP), such as dideoxyCytidine.

In the methods in which the IPC is susceptible to nuclease activity, the(substrate) nucleic acid molecule may be modified so as to protect itfrom nuclease activity. Suitable modifications are discussed herein andmay be selected from incorporation of methylation, protection of the 3′and/or 5′ ends, incorporation of synthetic nucleotides. Examples ofsynthetic nucleotides comprise phosphorothioate nucleotides and/orlocked nucleic acid nucleotides. Preferably, the synthetic nucleotidesare phosphorothioate nucleotides.

The various developments of the basic assay format can advantageously becombined to produce a particularly specific and sensitive method.Accordingly, the invention further provides a method of detecting theabsence or presence of a micro-organism in a sample, the samplecontaining a non-micro-organism source of nucleic acid modifyingactivity comprising:

-   -   (i) incubation of the sample with a reagent that lyses        non-microorganisms if present in the sample but does not lyse        microorganisms in the sample    -   (ii) optionally separation of the lysed cell material from the        intact microorganisms (if any) in the sample    -   (iii) contacting the (separated) intact microorganisms (if any)        in the sample with a high pH reagent and incubating for no more        than 5 minutes in order to inhibit the non-micro-organism source        of nucleic acid modifying activity (whilst not affecting the        nucleic acid modifying activity of the microorganism in the        sample)    -   (iv) adding a pH lowering reagent in order to stop the        incubation at high pH    -   (v) separation of the microorganisms if present in the sample        from the pH modifying reagents    -   (vi) lysis of any separated microorganisms    -   (vii) contacting the sample with a nucleic acid molecule which        acts as a substrate for nucleic acid modifying activity of the        micro-organism in the sample,    -   (viii) incubating the thus contacted sample under conditions        suitable for nucleic acid modifying activity; and    -   (ix) specifically determining the absence or presence of a        modified nucleic acid molecule resulting from the action of the        nucleic acid modifying activity on the substrate nucleic acid        molecule to indicate the absence or presence of the        microorganism, wherein the nucleic acid molecule is modified so        as to protect it from nuclease activity.

Step (ii) is an optional step because, in some embodiments, the lysedcell material does not need to be separated from the intactmicroorganisms. This is because step (iii) is used to inhibit thenucleic acid modifying activity found in the lysed cell material in anycase.

Similarly, the invention further provides a method of detecting theabsence or presence of a microorganism in a sample, the samplecontaining a non-microorganism source of nucleic acid modifying activitycomprising:

-   -   (a) incubation of the sample with a reagent that lyses        non-microorganisms if present in the sample but does not lyse        micro-organisms in the sample    -   (b) centrifugation of the sample to form a pellet containing        microorganisms if present in the sample    -   (c) removal of the supernatant from the pellet    -   (d) re-suspending the pellet in a high pH reagent and incubating        for no more than 5 minutes in order to inhibit the        non-micro-organism source of nucleic acid modifying activity        (whilst not affecting the nucleic acid modifying activity of the        microorganism in the sample)    -   (e) adding a pH lowering reagent in order to stop the incubation        at high pH    -   (f) a second centrifugation of the sample to form a pellet        containing micro-organisms if present in the sample    -   (g) removal of the supernatant from the pellet    -   (h) lysing any micro-organisms in the pellet    -   (i) contacting the sample with a nucleic acid molecule which        acts as a substrate for nucleic acid modifying activity of the        micro-organism in the sample,    -   (j) incubating the thus contacted sample under conditions        suitable for nucleic acid modifying activity; and    -   (k) specifically determining the absence or presence of a        modified nucleic acid molecule resulting from the action of the        nucleic acid modifying activity on the substrate nucleic acid        molecule to indicate the absence or presence of the        micro-organism, wherein the nucleic acid molecule is modified so        as to protect it from nuclease activity.

Thus, these methods represent a combination of the other methodsdescribed herein. Accordingly, all relevant embodiments apply to thisoverall method mutatis mutandis. For example, as for other embodimentsin which the nucleic acid is modified, the modification may be selectedfrom incorporation of methylation, protection of the 3′ and/or 5′ ends,incorporation of synthetic nucleotides. The synthetic nucleotides maycomprise phosphorothioate nucleotides and/or locked nucleic acidnucleotides. Preferably, the synthetic nucleotides are phosphorothioatenucleotides.

In particular embodiments, the nucleic acid molecule is added to thesample at a concentration of at least 2 nM and less than 50 nM (e.g. 2nM to 25 nM, 5 nM to 15 nM, or 7.5 to 12.5 nM), such as 2 nM, 5 nM, 7.5nM or 10 nM. The nucleic acid molecule may be included in the lysismixture used in step (vi) or (h) of the method (i.e. the specifiedconcentration is the concentration in the lysis mixture). Thus, steps(vi) and (vii) or (h) and (i) respectively may effectively be combinedas a single step in some embodiments. The lysis mixture may be asspecified in Table 1 or as discussed elsewhere in this disclosure.

The method may similarly comprise adding to the sampledeoxyribonucleotide triphosphates at a concentration of more than 50 μM,preferably at least 100 μM, such as 55 to 300 μM, or 60 to 250 μM, or 75to 200 μM. Again, the dNTPs may be included in the lysis mixture used instep (vi) or (h) of the method (i.e. the specified concentration is theconcentration in the lysis mixture). Thus, steps (vi) and (vii) or (h)and (i) respectively may effectively be combined as a single step insome embodiments.

As discussed in greater detail hereinabove, the high pH reagent may beor comprise NaOH or Na2CO3. In specific embodiments, the concentrationof the high pH reagent is around 5 mM or greater. The pH loweringreagent may comprise a buffer or an acid, such as a Tris-HCl buffer(e.g. pH 7.2 or 8). In specific embodiments, step (iii) or (d) isperformed at a temperature between 15 and 30 degrees Celsius or isperformed at room temperature. Each and/or all steps of the method maybe performed at a temperature between 15 and 30 degrees Celsius or atroom temperature in some embodiments. Where nucleic acid amplificationsteps such as PCR are utilised, those steps will need to be performed atappropriate temperatures as detailed herein and understood by theskilled person.

In specific embodiments, a nuclease susceptible IPC may be employed.Thus, step (vii) or (i) respectively may comprise contacting the samplewith a nucleic acid molecule which acts as a substrate for nucleic acidmodifying activity of the micro-organism in the sample together with aninternal positive control (IPC) nucleic acid molecule, wherein the IPCnucleic acid molecule is susceptible to nuclease activity and is used toidentify contaminating nuclease activity in the pellet.

Alternatively, a nuclease resistant IPC may be employed. Thus, step(vii) or (i) respectively may comprise contacting the sample with anucleic acid molecule which acts as a substrate for nucleic acidmodifying activity of the micro-organism in the sample together with aninternal positive control (IPC) nucleic acid molecule, wherein the IPCnucleic acid molecule is modified so as to protect it from nucleaseactivity. Suitable modifications are discussed in greater detail hereinand may be selected from incorporation of methylation, protection of the3′ and/or 5′ ends, incorporation of synthetic nucleotides. The syntheticnucleotides may be or comprise phosphorothioate nucleotides and/orlocked nucleic acid nucleotides. Preferably, the synthetic nucleotidesare phosphorothioate nucleotides. If both molecules are modified, it ispreferable that they are modified in the same or similar manner suchthat the nuclease resistance is comparable. This permits the IPC toperform a most useful comparator role to determine the impact ofnuclease activity on the substrate molecule.

As already mentioned, in some embodiments, steps (vi) and (vii) or (h)and (i) are performed together. Here, the nucleic acid molecule is addedto the sample together with a lysis reagent to form a lysis mixture.

As discussed in further detail herein, which discussion applies mutatismutandis, the detection of the modified nucleic acid molecule may bedetected by a range of methods including by sequencing or nucleic acidamplification. In specific embodiments, step (ix) or (k) respectivelycomprises a nucleic acid amplification step.

Any suitable nucleic acid molecule may be employed. In some embodiments,the nucleic acid molecule incorporates uracil residues. In specificembodiments, the nucleic acid molecule is at least partially doublestranded and comprises uracil residues in the complementary strand. Insuch embodiments, the methods (and in particular step (ix) or (k) of therespective method) may comprise adding Uracil DNA Glycosylase (UDG) tothe sample in order to degrade the uracil residues in the complementarystrand. In further embodiments, the complementary strand of the nucleicacid molecule comprises a modification at the 3′ end to preventextension. By “extension” is meant the addition of further nucleotides.Any suitable modification may be employed. In specific embodiments, themodification is or comprises incorporation of a non-extendiblenucleotide. Any suitable non-extendible nucleotide may be employed. Forexample, the non-extendible nucleotide may be or comprise a dideoxynucleotide triphosphate (ddNTP), such as dideoxyCytidine.

In certain embodiments, the IPC nucleic acid molecule comprisesidentical primer binding sites to the nucleic acid molecule such thatthere is competition for primer binding during the step of detection ofthe modified nucleic acid molecule (in step (ix) or (k)).

As discussed herein there are a range of amplification technologiesavailable, many of which rely upon probes (such as hydrolytic or hairpinprobes). Thus, in some embodiments the methods include use of a probe,in particular in step (ix) or (k). In specific embodiments, a nucleicacid probe is added in step (ix) or (k) of the method. This probe bindsto a target probe sequence within the (sense strand of the) nucleic acidmolecule. By “binds” is meant hybridization under the conditions appliedto the method as would be readily appreciated by one skilled in the art.In some embodiments, a further nucleic acid probe is utilised, forexample added in step (ix) or (k), which binds to a target probesequence within the IPC nucleic acid molecule. In specific embodiments,the nucleic acid probe does not bind to the IPC nucleic acid moleculeand the further nucleic acid probe does not bind to the (substrate)nucleic acid molecule. The probes and nucleic acid molecules (IPC orsubstrate) can be designed to avoid unwanted cross-hybridization usingtechniques and tools (such as online design tools) known in the art.

The nucleic acid probe and/or further nucleic acid probe may belabelled. In specific embodiments, the nucleic acid probe and furthernucleic acid probe are differently labelled. For example, they may belabelled with fluorophores which have different wavelengths of maximalemission. Suitable pairs of labels can be readily selected by oneskilled in the art, for example FAM and Texas Red may be used asdifferent labels.

According to all aspects of the invention, the nucleic acid modifyingactivity may be any activity that is useful for indicating microorganismviability. The nucleic acid modifying activity is an enzymatic activityprovided by the microorganism. Examples include polymerase and/or ligaseactivity. Preferably, the nucleic acid modifying activity is polymeraseactivity. Polymerase activity may comprise DNA and/or RNA polymeraseactivity. Preferably the polymerase activity is DNA and/or RNApolymerase activity. Ligase activity may be ATP or NAD dependent. Othernucleic acid modifying activities relevant to viability mayalternatively be measured such as phosphatase, kinase and/or nucleaseactivity.

Preferably, the action of the nucleic acid modifying activity on thesubstrate nucleic acid molecule produces an extended nucleic acidmolecule.

Suitable substrate molecules are described herein in detail. Referencecan also be made to WO2011/130584, WO2010/119270 and WO2009/007719 (thepertinent disclosures of which are hereby incorporated) where suitablesubstrate molecules useful for detecting nucleic acid modifying activityare disclosed. In the case of phosphatase activity, suitable nucleicacid molecules are disclosed in WO2006/123154, which disclosure ishereby incorporated by reference.

The substrate nucleic acid molecules for use in the methods, andinclusion in the kits, of the invention, must be of sequence andstructure such that the NAD− dependent ligase can act on the molecule toproduce a detectable ligated (novel) nucleic acid molecule.

Suitable substrate nucleic acid molecules for use in the invention aredescribed in more detail in the experimental section below. Thus, thesubstrate may be comprised of the following molecules:

AS (SEQ ID NO: 6) Uaggcgucggugacaaacggccagcguuguugucucu-DDC (3′terminal is a dideoxy-C) S1 (SEQ ID NO: 7)Gccgatatcggacaacggccgaactgggaaggcgagactgaccgaccgataagctagaacagagagacaacaac

This is an example of a substrate nucleic acid molecule whichincorporates uracil residues in the antisense strand. The nucleic acidmolecule is partially double stranded and comprises uracil residues inthe complementary strand. This permits Uracil DNA Glycosylase (UDG) todegrade the uracil residues in the complementary strand followingextension and thus prevents the substrate molecule from beingnon-specifically amplified in the absence of extension (i.e. in theabsence of nucleic acid modifying activity in the sample). In furtherembodiments, the complementary strand of the nucleic acid moleculecomprises a modification at the 3′ end to prevent extension. By“extension” is meant the addition of further nucleotides. Any suitablemodification may be employed. In specific embodiments, the modificationis or comprises incorporation of a non-extendible nucleotide. Anysuitable non-extendible nucleotide may be employed. For example, thenon-extendible nucleotide may be or comprise a dideoxy nucleotidetriphosphate (ddNTP), such as dideoxyCytidine as shown in SED ID NO: 6.

It is noted that variants of these sequences may be utilised in thepresent invention. For example, additional flanking sequences may beadded. Alternative ddNTPs may be employed. Variant sequences may have atleast 90%, at least 91%, at least 92%, at least 93%, at least 94%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%nucleotide sequence identity with the nucleotide sequences of thesubstrate nucleic acid molecules set forth as SEQ ID NOs 6 and 7. Thenucleic acid molecules may incorporate synthetic nucleotide analogues asappropriate or may be RNA or PNA based for example, or mixtures thereof.Suitable modifications, for example, to protect from nuclease activity,are described herein. They may be labelled, such as using a fluorescentlabel, or FRET pair, in certain embodiments to facilitate detection.Suitable detection methods are described herein.

Thus, the (substrate) nucleic acid molecules include any natural nucleicacid and natural or synthetic analogues that are capable of being actedupon by nucleic acid modifying activity in order to generate a (noveldetectable) nucleic acid molecule. The substrate may be extended and/orligated in specific embodiments. Combinations of nucleic acid substratemolecules may be employed to permit detection of polymerase and ligaseactivity in some embodiments.

Preferably, the nucleic acid substrate is present in excess, and inparticular in large molar excess, over the nucleic acid modifyingactivity (provided by the microorganisms) in the sample. This is animportant technical distinction over prior art methods. Because a novelextended or ligated nucleic acid molecule is detected, only the presenceof this molecule in the sample is essential for the detection methods towork effectively. Thus, it is not detrimental to the methods of theinvention if other nucleic acid molecules are present in the sample suchas from the microorganisms to be detected or from mammalian or othersources which may be found in the sample to be tested for example.

In some embodiments, the substrate and/or primers may incorporatecomplementary non-naturally occurring molecules which can base pair witheach other, to avoid non-specific detection of genomic DNA. As anexample, pyDAD and puADA may be incorporated into primers and substratemolecules as appropriate (Sismour et al., Nucleic Acids Research, 2004,Vol. 32, No. 2: 728-735).

As also discussed herein, the methods of the invention may incorporatean IPC molecule. Any suitable IPC may be employed according to therequirements of the method. As an example, the following IPC may be usedin the invention:

(SEQ ID NO: 3) gcc gat atc gga caa cgg ccg aac tgg gaa ggc gagatc agc agg cca cac gtt aaa gac aga gag aca acaacg ctg gcc gtt tgt cac cga cgc cta

In all methods of the invention specifically determining the absence orpresence of the modified nucleic acid molecule may comprise, consistessentially of or consist of a nucleic acid amplification step. Thisserves to make the methods of the invention maximally sensitive. Suchamplification techniques are well known in the art, and include methodssuch as PCR, NASBA (Compton, 1991), 3SR (Fahy et al., 1991), Rollingcircle replication, Transcription Mediated Amplification (TMA), stranddisplacement amplification (SDA) Clinical Chemistry 45: 777-784, 1999,the DNA oligomer self-assembly processes described in U.S. Pat. No.6,261,846 (incorporated herein by reference), ligase chain reaction(LCR) (Barringer et al., 1990), selective amplification of targetpolynucleotide sequences (U.S. Pat. No. 6,410,276), arbitrarily primedPCR (WO 90/06995), consensus sequence primed PCR (U.S. Pat. No.4,437,975), invader technology, strand displacement technology and nickdisplacement amplification (WO 2004/067726). The list above is notintended to be exhaustive. Any nucleic acid amplification technique maybe used provided the appropriate nucleic acid product is specificallyamplified. Similarly, sequencing based methodologies may be employed insome embodiments to include any of the range of next generationsequencing platforms.

Amplification is achieved with the use of amplification primers specificfor the sequence of the modified nucleic acid molecule which is to bedetected. In order to provide specificity for the nucleic acid moleculesprimer binding sites corresponding to a suitable region of the sequencemay be selected. The skilled reader will appreciate that the nucleicacid molecules may also include sequences other than primer bindingsites which are required for detection of the novel nucleic acidmolecule produced by the modifying activity in the sample, for exampleRNA Polymerase binding sites or promoter sequences may be required forisothermal amplification technologies, such as NASBA, 3SR and TMA.

One or more primer binding sites may bridge the ligation/extensionboundary of the substrate nucleic acid molecule such that anamplification product is only generated if ligation/extension hasoccurred, for example. Alternatively, primers may bind either side ofthe ligation/extension boundary and direct amplification across theboundary such that an amplification product is only generated(exponentially) if the ligated/extended nucleic acid molecule is formed.Primers and the substrate nucleic acid molecule(s) may be designed toavoid non-specific amplification (e.g. of genomic DNA in the sample).

Suitable primers for use in the methods of the invention are set forthin the experimental section below. They include primers comprising,consisting essentially of or consisting of SEQ ID NO: 4 and/or 5. Theseprimers form a separate aspect of the invention. It is noted thatvariants of these sequences may be utilised in the present invention. Inparticular, additional sequence specific flanking sequences may beadded, for example to improve binding specificity, as required. Variantsequences may have at least 90%, at least 91%, at least 92%, at least93%, at least 94%, at least 95%, at least 96%, at least 97%, at least98%, or at least 99% nucleotide sequence identity with the nucleotidesequences of the primers set forth in the experimental section. Theprimers may incorporate synthetic nucleotide analogues as appropriate ormay be RNA or PNA based for example, or mixtures thereof. The primersmay be labelled, such as with fluorescent labels and/or FRET pairs,depending upon the mode of detection employed. Probes may be utilised,again which may be labelled, as desired.

Thus, in certain aspects, the methods of the invention are carried outusing nucleic acid amplification techniques in order to detect themodified nucleic acid molecule produced as a direct result of the actionof nucleic acid-modifying activity on the substrate nucleic acidmolecule which indicates the presence of a micro-organism in the sample.In certain embodiments the technique used is selected from PCR, NASBA,3SR, TMA, SDA and DNA oligomer self-assembly.

Detection of the amplification products may be by routine methods, suchas, for example, gel electrophoresis but in some embodiments is carriedout using real-time or end-point detection methods.

A number of techniques for real-time or end-point detection of theproducts of an amplification reaction are known in the art. Theseinclude use of intercalating fluorescent dyes such as SYBR Green I(Sambrook and Russell, Molecular Cloning—A Laboratory Manual, Thirdedition), which allows the yield of amplified DNA to be estimated basedupon the amount of fluorescence produced. Many of the real-timedetection methods produce a fluorescent read-out that may becontinuously monitored; specific examples including molecular beaconsand fluorescent resonance energy transfer probes. Real-time andend-point techniques are advantageous because they keep the reaction ina “single tube”. This means there is no need for downstream analysis inorder to obtain results, leading to more rapidly obtained results.Furthermore keeping the reaction in a “single tube” environment reducesthe risk of cross contamination and allows a quantitative output fromthe methods of the invention. This may be particularly important in thecontext of the present invention where health and safety concerns may beof paramount importance (such as in detecting potential microbialinfection in a patient samples for example).

Real-time and end-point quantitation of PCR reactions may beaccomplished using the TaqMan® system (Applied Biosystems), see Hollandet al; Detection of specific polymerase chain reaction product byutilising the 5′-3′ exonuclease activity of Thermus aquaticus DNApolymerase; Proc. Natl. Acad. Sci. USA 88, 7276-7280 (1991), Gelmini etal. Quantitative polymerase chain reaction-based homogeneous assay withflurogenic probes to measure C-Erb-2 oncogene amplification. Clin. Chem.43, 752-758 (1997) and Livak et al. Towards fully automated genome widepolymorphism screening. Nat. Genet. 9, 341-342 (19995) (incorporatedherein by reference). This type of probe may be generically referred toas a hydrolytic probe. Suitable hydrolytic/Taqman probes for use in realtime or end point detection are also provided. The probe may be suitablylabelled, for example using the labels detailed below.

In the Molecular Beacon system, see Tyagi & Kramer. Molecularbeacons—probes that fluoresce upon hybridization. Nat. Biotechnol. 14,303-308 (1996) and Tyagi et al. Multicolor molecular beacons for allelediscrimination. Nat. Biotechnol. 16, 49-53 (1998) (incorporated hereinby reference), the beacons are hairpin-shaped probes with an internallyquenched fluorophore whose fluorescence is restored when bound to itstarget. These probes may be referred to as hairpin probes. Suitableprobes useful in the present invention are set forth as SEQ ID NO: 1 and2.

A further real-time fluorescence based system which may be incorporatedin the methods of the invention is the Scorpion system, see Detection ofPCR products using self-probing amplicons and fluorescence by Whitcombeet al. Nature Biotechnology 17, 804-807 (1 Aug. 1999). Additionalreal-time or end-point detection techniques which are well known tothose skilled in the art and which are commercially available includeLightcycler® technology, Amplifluour® primer technology, DzyNA primers(Todd et al., Clinical Chemistry 46:5, 625-630 (2000)), or the Plexor™qPCR and qRT-PCR Systems.

Thus, in further aspects of the invention the products of nucleic acidamplification are detected using real-time or end point techniques. Inspecific embodiments of the invention the real-time technique consistsof using any one of hydrolytic probes (the Taqman® system), FRET probes(Lightcycler® system), hairpin primers (Amplifluour® system), hairpinprobes (the Molecular beacons system), hairpin probes incorporated intoa primer (the Scorpion® probe system), primers incorporating thecomplementary sequence of a DNAzyme and a cleavable fluorescent DNAzymesubstrate (DzYNA), Plexor qPCR and oligonucleotide blocking systems.

Amplification products may be quantified to give an approximation of themicrobial nucleic acid modifying activity in the sample and thus thelevel of microorganisms in the sample. Thus, “absence or presence” isintended to encompass quantification of the levels of microorganisms inthe sample.

In certain embodiments, the reaction mixture will contain all of; thesample under test, the substrate nucleic acid molecule(s), reagents,buffers and enzymes required for amplification of the modified nucleicacid molecule optionally in addition to the reagents required to allowreal time or end-point detection of amplification products. Thus theentire detection method for the nucleic acid modifying activity (fromthe one or more bacterial cells or micro-organisms of interest) mayoccur in a single reaction, with a quantitative output, and without theneed for any intermediate washing steps. Use of a “single tube” reactionis advantageous because there is no need for downstream analysis inorder to obtain results, leading to more rapidly obtained results.Furthermore keeping the reaction in a “single tube” environment reducesthe risk of cross contamination and allows a quantitative output fromthe methods of the invention. Also, single tube reactions are moreamenable to automation, for example in a high throughput context.

Alternatively, the methods of the invention may be carried out instep-wise fashion. Thus, in a first step it may first be necessary toprepare the sample in a form suitable for use in the method of theinvention. For example, as discussed herein, selective cell lysis orincreasing cellular permeability may be required. Capture of specificnucleic acid modifying activity, such as polymerase or ligase, may alsobe desirable again as described herein. Other (sources of) nucleic acidmodifying activity, such as nuclease activity, may be inhibited etc.

The steps of the methods of the invention prior to the amplificationstep may not comprise a step performed at a temperature of more than 40°C., more than 50° C., more than 60° C., more than 70° C., more than 80°C., more than 90° C. or more than 95° C.

Alternatively, the methods of the invention may not comprise any stepsperformed at a temperature of more than 40° C., more than 50° C., morethan 60° C., more than 70° C., more than 80° C., more than 90° C. ormore than 95° C.

The steps of the methods of the invention prior to the amplificationstep may be performed at a temperature between 10 and 50 degreesCelsius, between 15 and 45 degrees Celsius, between 20 and 40 degreesCelsius, between 25 and 40 degrees Celsius, between 30 and 40 degreesCelsius, between 25 and 35 degrees Celsius, or between 15 and 30 degreesCelsius, optionally the steps of the methods prior to the amplificationstep may be performed at room temperature.

Alternatively, steps of the methods of the invention may all beperformed at a temperature between 10 and 50 degrees Celsius, between 15and 45 degrees Celsius, between 20 and 40 degrees Celsius, between 25and 40 degrees Celsius, between 30 and 40 degrees Celsius, between 25and 35 degrees Celsius, or between 15 and 30 degrees Celsius, optionallyall of the steps of the methods may be performed at room temperature.

The methods of the invention may comprise a step of inactivatingnuclease activity in the sample. Alternatively, the methods of theinvention do not comprise a step of inactivating nuclease activity inthe sample. If performed, the step of inactivating nuclease activitytakes place after the incubation step and before the step ofspecifically determining the absence or presence of a modified nucleicacid molecule (e.g. by amplification).

A “sample” in the context of the present invention is defined to includeany sample in which it is desirable to test for the presence of amicroorganism, such as a fungus (e.g. a yeast) or a bacterium,expressing nucleic acid modifying activity. Thus the sample maycomprise, consist essentially of or consist of a clinical sample, suchas a blood sample. The methods of the invention are particularlyapplicable to the rapid determination of negative blood cultures. Thus,the sample may comprise a blood culture sample from a patient suspectedof suffering from, or being screened for, a bloodstream infection. Thesample may be any suitable volume such as 1 to 10 ml, preferably a 1 mlblood culture sample.

Alternatively the sample may be or comprise an in vitro assay system forexample. Samples may comprise, consist essentially of or consist ofbeverage or food samples or preparations thereof, or pharmaceutical orcosmetic products such as personal care products including shampoos,conditioners, moisturisers etc., all of which are tested for microbialcontamination as a matter of routine. The sample may comprise, consistessentially of or consist of tissue or cells and may comprise, consistessentially of or consist of a sputum or a blood sample or a plateletsample for example. In addition, the methods and kits of the inventionmay be used to monitor contamination of surfaces, such as for example inlocations where food is being prepared. Contamination is indicated bythe presence of microbial nucleic acid modifying activity. Thecontamination may be from any microbial source, in particular bacterialor fungal (e.g yeast) contamination. Furthermore, the invention is alsouseful in monitoring environmental conditions such as water supplies,wastewater, marine environments etc. The invention is also useful inmonitoring bacterial growth in fermentation procedures and in airsampling where bacteria or spore content can be assessed in hospital,industrial facilities or in biodefence applications.

The methods of the invention have various utilities in addition toscreening samples for the absence or presence of a microorganism.Accordingly, in a further aspect the invention provides for use of amethod as described herein for screening for resistance of amicro-organism to an agent directed against micro-organism. The methodmay involve steps of exposing the sample containing the microorganism ofinterest to the agent and then performing a method of the invention todetermine whether the microorganism is resistant. If the microorganismis resistant, the modified nucleic acid molecule will be detected.Typically such methods are performed using well characterised samples,such as a cultured clinical isolate of a microorganism of interest.

Similarly, the invention provides for use of a method as describedherein for screening candidate agents which may be capable of killing orpreventing growth of one or more micro-organisms. This method mayinvolve exposing the sample containing the microorganism to the agentand then performing a method of the invention. If the agent is aneffective killing agent, there would be no (or reduced) modified nucleicacid detected. Typically such methods are performed using wellcharacterised samples, such as a cultured clinical isolate of amicroorganism of interest. The methods may be performed as a time courseexperiment to determine whether the agent is able to prevent growth ofthe microorganism (even if not able to kill). There may be a parallelreaction run in the absence of the agent to determine the growth of themicroorganism in the absence of the agent. This provides a comparisonfor the effectiveness of the agent in terms of growth inhibitionactivity.

Further, the invention provides for use of the method as describedherein for diagnosing an infection, or a disease associated with thepresence of a micro-organism in a subject. In this context the “sample”will generally be a clinical sample. The sample being used will dependon the condition that is being tested for. Typical samples which may beused, but which are not intended to limit the invention, include wholeblood, serum, plasma, platelet and urine samples etc. taken from apatient, most preferably a human patient. In a preferred embodiment, thetest will be an in vitro test carried out on a sample removed from asubject. In a further embodiment, the above-described diagnostic methodsmay additionally include the step of obtaining the sample from asubject. Methods of obtaining a suitable sample from a subject are wellknown in the art. Alternatively, the method may be carried out beginningwith a sample that has already been isolated from the patient in aseparate procedure. The diagnostic methods will most preferably becarried out on a sample from a human, but the method of the inventionmay have diagnostic utility for many animals.

The diagnostic methods of the invention may be used to complement anyalready available diagnostic techniques, potentially as a method ofconfirming an initial diagnosis. Alternatively, the methods may be usedas a preliminary diagnosis method in their own right, since the methodsprovide a quick and convenient means of diagnosis. Furthermore, due totheir inherent sensitivity, the diagnostic methods of the inventionrequire only a minimal sample, thus preventing unnecessary invasivesurgery. Also, a large but non-concentrated sample may also be testedeffectively according to the methods of the invention.

Thus, the methods of the invention have multiple applications beyonddetection of contaminating organisms in a sample. The descriptionprovided above with respect to the various aspects of the inventionapplies mutatis mutandis to the other aspects of the invention and isnot repeated for reasons of conciseness. For example, suitable controlsmay be incorporated for each method of the invention.

In specific embodiments the microorganism is a pathogenic microorganism,such as a pathogenic bacterium. The bacterium may be any bacterium whichis capable of causing infection or disease in a subject, preferably ahuman subject. In one embodiment, the bacteria comprises or consistsessentially of or consists of any one or more of Staphylococcus species,in particular Staphylococcus aureus and preferably methicillin resistantstrains, Enterococcus species, Streptococcus species, Mycobacteriumspecies, in particular Mycobacterium tuberculosis, Vibrio species, inparticular Vibrio cholerae, Salmonella and/or Escherichia coli etc. Thebacteria may comprise, consist essentially of or consist of Clostridiumspecies and in particular C. difficile in certain embodiments. C.difficile is the major cause of antibiotic-associated diarrhoea andcolitis, a healthcare associated intestinal infection that mostlyaffects elderly patients with other underlying diseases. Candida speciessuch as C. albicans, C. parapsilosis and C. glabrata may be detected.Cryptococcus species such as C. neoformans may be detected. Fungaemiasuch as Candidaemia may be detected (presence or absence) using theinvention.

In certain embodiments, according to these further aspects of theinvention, the molecule which is being tested in the method (either forresistance or ability to treat an infection or toxicity to cells) is anantimicrobial compound. In the compound screening methods, any moleculemay be tested. Examples include antimicrobial agents, nucleic acidmolecules including siRNA (dsRNA) molecules and antisense molecules,small molecules, antibodies and all derivatives thereof including Fabfragments, variable region fragments and single domain antibodies forexample provided they retain binding affinity etc. The method may becarried out in a high throughput context to screen large numbers ofmolecules in a short period of time.

The antimicrobial agent, in one embodiment, may be taken from the twomain types of antimicrobial agents, antibiotics (natural substancesproduced by micro-organisms) and chemotherapeutic agents (chemicallysynthesized), or may be a hybrid of the two such as semi-syntheticantibiotics (a subsequently modified naturally produced antibiotic) orsynthetic antibiotics (synthesised versions of natural antibiotics).

Suitable candidate antimicrobial agents may, following a positive resultin the methods of the invention in terms of ability to kill or preventgrowth of a bacterium or bacterial cell or other suitable micro-organismbe tested for at least one or more of the following properties:

-   -   (1) the agent should be non-toxic to the subject and without        adverse side effects,    -   (2) the agent should be non-allergenic to the subject,    -   (3) the agent should not eliminate the natural flora of the        subject,    -   (4) the agent should be stable,    -   (5) the agent should preferably be cheap and readily        available/easy to manufacture; and    -   (6) the agent should be sufficiently potent that pathogen        resistance does not develop (to any appreciable degree). This        feature may be tested according to the methods described above.

In one embodiment, a combination of multiple suitable antimicrobialagents may be tested for ability to treat an infection and/or forresistance thereto.

Antibiotics or derivatives thereof which may be tested for resistanceand perhaps also for their novel ability to treat certain infections maybe selected from the following groups, provided by way of example andnot limitation; beta-lactams such as penicillin, in particularpenicillin G or V, and cephalosporins such as cephalothin,semi-synthetic penicillins such as ampicillin, methicillin andamoxicillin, clavulanic acid preferably used in conjunction with asemi-synthetic penicillin preparation (such as clavamox or augmentin forexample), monobactams such as aztreonam, carboxypenems such as imipenem,aminoglycosides such as streptomycin, kanamycin, tobramycin andgentamicin, glycopeptides such as vancomycin, lincomycin andclindamycin, macrolides such as erythromycin and oleandomycin,polypeptides such as polymyxin and bacitracin, polyenes such asamphotericin and nystatin, rifamycins such as rifampicin, tetracyclinessuch as tetracycline, semi-synthetic tetracyclines such as doxycycline,chlor tetracycline, chloramphenicol, quinolones such as nalidixic acidand fluoroquinolone and competitive inhibitors such as sulfonamides, forexample gantrisin and trimethoprim. Ceftriaxone and/or nitroflurazonemay also be utilised.

Still further the invention provides for use of a method as describedherein for detecting the presence of microorganism contamination in aplatelet containing sample. In such aspects, the methods may incorporatesub-steps of:

-   -   (i) lysis of the platelets under conditions that leave the        microorganism cells intact. This principally allows selective        concentration of microorganisms prior to testing for the        presence of nucleic acid modifying activity. Thus, nucleic acid        modifying activity provided by mammalian cells can be removed        prior to testing    -   (ii) concentration of the microorganisms (for example by        centrifugation to produce a bacterial cell containing pellet)    -   (iii) lysis of the microorganisms or a treatment to increase the        permeability of the microorganisms to release the nucleic acid        modifying activity.

The invention also relates to kits useful in performing the methods ofthe invention. Thus, there is provided a kit for carrying out a methodas described herein comprising:

-   -   (a) at least one nucleic acid molecule which acts as a substrate        for nucleic acid modifying activity of the micro-organism in the        sample, wherein the at least one nucleic acid molecule is at        least partially double stranded and comprises uracil residues in        the complementary strand, characterised in that the nucleic acid        molecule is modified so as to protect it from nuclease activity    -   (b) at least one internal positive control (IPC) nucleic acid        molecule which comprises identical primer binding sites to the        nucleic acid molecule such that there is competition for primer        binding in a nucleic acid amplification reaction containing both        the nucleic acid molecule and the IPC.

The kits may incorporate any of the components required to perform themethods of the invention. Accordingly, all discussion of the methods ofthe invention applies mutatis mutandis.

In the context of the present invention, the nucleic acid molecule ispre-modified so as to protect it from nuclease activity.

In some embodiments, the kit further comprises a nucleic acid probewhich binds to a target probe sequence within the (sense strand of the)nucleic acid molecule. The kit may further comprise a further nucleicacid probe which binds to a target probe sequence within the IPC nucleicacid molecule. In certain embodiments, the nucleic acid probe does notbind to the IPC nucleic acid molecule and the further nucleic acid probedoes not bind to the nucleic acid molecule. The nucleic acid probeand/or further nucleic acid probe may be labelled. In specificembodiments, the nucleic acid probe and further nucleic acid probe aredifferently labelled. For example, they may be labelled withfluorophores which have different wavelengths of maximal emission.Suitable pairs of labels can be readily selected by one skilled in theart, for example FAM and Texas Red may be used as different labels.

In further embodiments, the complementary strand of the nucleic acidmolecule comprises a modification at the 3′ end to prevent extension. By“extension” is meant the addition of further nucleotides. Any suitablemodification may be employed. In specific embodiments, the modificationis or comprises incorporation of a non-extendible nucleotide. Anysuitable non-extendible nucleotide may be employed. For example, thenon-extendible nucleotide may be or comprise a dideoxy nucleotidetriphosphate (ddNTP), such as dideoxyCytidine.

In further embodiments, the IPC is modified so as to protect it fromnuclease activity. In the context of the present invention, the IPC ispre-modified so as to protect it from nuclease activity. Suitablemodifications are discussed in greater detail herein and may be selectedfrom incorporation of methylation, protection of the 3′ and/or 5′ ends,incorporation of synthetic nucleotides. The synthetic nucleotides may beor comprise phosphorothioate nucleotides and/or locked nucleic acidnucleotides. Preferably, the synthetic nucleotides are phosphorothioatenucleotides. If both molecules are modified, it is preferable that theyare modified in the same or similar manner such that the nucleaseresistance is comparable. This permits the IPC to perform a most usefulcomparator role to determine the impact of nuclease activity on thesubstrate molecule.

In further embodiments, the kit further comprises a high pH reagent. Thehigh pH reagent may be or comprise NaOH or Na2CO3. In specificembodiments, the concentration of the high pH reagent is around 5 mM orgreater. The kit may further comprise a pH lowering agent. The pHlowering reagent may comprise a buffer or an acid, such as a Tris-HClbuffer (e.g. pH 7.2 or 8).

The kits may incorporate a suitable carrier in which the reactions takeplace. Advantageously, such a carrier may comprise a multi-well plate,such as a 48 or 96 well plate for example. Such a carrier allows thedetection methods to be carried out in relatively small volumes—thusfacilitating scale up and minimising the sample volume required.

The kits will typically incorporate suitable instructions. Theseinstructions permit the methods of the invention to be carried outreliably using the kits of the invention.

The invention may be further defined in the following set of numberedclauses:

A method of detecting the absence or presence of a micro-organism in asample comprising:

-   -   (a) contacting the sample with a nucleic acid molecule which        acts as a substrate for nucleic acid modifying activity of the        micro-organism in the sample,    -   (b) incubating the thus contacted sample under conditions        suitable for nucleic acid modifying activity; and    -   (c) specifically determining the absence or presence of a        modified nucleic acid molecule resulting from the action of the        nucleic acid modifying activity on the substrate nucleic acid        molecule to indicate the absence or presence of the        micro-organism, characterised in that the nucleic acid molecule        is modified so as to protect it from nuclease activity.

2. The method of clause 1 wherein the modification is selected fromincorporation of methylation, protection of the 3′ and/or 5′ ends andincorporation of synthetic nucleotides.

3. The method of clause 2 wherein the synthetic nucleotides comprisephosphorothioate nucleotides and/or locked nucleic acid nucleotides.

4. A method of detecting the absence or presence of a micro-organism ina sample comprising:

-   -   (a) contacting the sample with a nucleic acid molecule which        acts as a substrate for nucleic acid modifying activity of the        micro-organism in the sample,    -   (b) incubating the thus contacted sample under conditions        suitable for nucleic acid modifying activity; and    -   (c) specifically determining the absence or presence of a        modified nucleic acid molecule resulting from the action of the        nucleic acid modifying activity on the substrate nucleic acid        molecule to indicate the absence or presence of the        micro-organism, characterised in that the nucleic acid molecule        is added to the sample at a concentration of at least 2 nM but        less than 50 nM.

5. The method of clause 4 wherein step (a) and/or (b) comprises addingto the sample deoxyribonucleotide triphosphates at a concentration of atleast 100 μM.

6. A method of detecting the absence or presence of a micro-organism ina sample, the sample containing a non-micro-organism source of nucleicacid modifying activity comprising:

-   -   (a) treating the sample under high pH conditions for no more        than 5 minutes in order to inhibit the non-micro-organism source        of nucleic acid modifying activity (whilst not affecting the        nucleic acid modifying activity of the micro-organism in the        sample),    -   (b) contacting the sample with a nucleic acid molecule which        acts as a substrate for nucleic acid modifying activity of the        micro-organism in the sample,    -   (c) incubating the thus contacted sample under conditions        suitable for nucleic acid modifying activity; and    -   (d) specifically determining the absence or presence of a        modified nucleic acid molecule resulting from the action of the        nucleic acid modifying activity on the substrate nucleic acid        molecule to indicate the absence or presence of the        micro-organism.

7. The method of clause 6 wherein the high pH conditions comprisecontacting the sample with NaOH or Na2CO3.

8. The method of clause 7 wherein the concentration of the NaOH orNa2CO3 is around 5 mM or greater.

9. The method of any one of clauses 6 to 8 wherein the treatment underhigh pH conditions is stopped by adding a reagent to lower the pH.

10. The method of clause 9 wherein the pH is lowered by adding a bufferor an acid.

11. The method of clause 10 wherein the buffer comprises a Tris-HClbuffer (e.g. pH 7.2 or 8).

12. The method of any one of clauses 6 to 11 wherein step (a) isperformed at a temperature between 15 and 30 degrees Celsius.

13. The method of any one of clauses 6 to 12 wherein step (a) isperformed at room temperature.

14. The method of any one of clauses 1 to 13 wherein the method isperformed at a temperature between 15 and 30 degrees Celsius.

15. The method of any one of clauses 1 to 14 wherein the method isperformed at room temperature.

16. A method of detecting the absence or presence of a micro-organism ina sample, the sample containing a non-micro-organism source of nucleicacid modifying activity comprising:

-   -   (a)    -   (i) incubation of the sample with a reagent that lyses        non-microorganisms if present in the sample but does not lyse        microorganisms in the sample    -   (ii) optionally separation of the lysed cell material from the        intact microorganisms (if any) in the sample    -   (iii) contacting the (separated) intact microorganisms (if any)        in the sample with a high pH reagent and incubating for no more        than 5 minutes in order to inhibit the non-micro-organism source        of nucleic acid modifying activity (whilst not affecting the        nucleic acid modifying activity of the micro-organism in the        sample)    -   (iv) adding a pH lowering reagent in order to stop the        incubation at high pH    -   (v) separation of the microorganisms if present in the sample        from the pH modifying reagents    -   (vi) lysis of any separated microorganisms    -   (vi) contacting the sample with a nucleic acid molecule which        acts as a substrate for nucleic acid modifying activity of the        micro-organism in the sample,    -   (vii) incubating the thus contacted sample under conditions        suitable for nucleic acid modifying activity; and    -   (viii) specifically determining the absence or presence of a        modified nucleic acid molecule resulting from the action of the        nucleic acid modifying activity on the substrate nucleic acid        molecule to indicate the absence or presence of the        micro-organism.

or

-   -   (b)    -   (i) incubation of the sample with a reagent that lyses        non-micro-organisms if present in the sample but does not lyse        micro-organisms in the sample    -   (ii) centrifugation of the sample to form a pellet containing        micro-organisms if present in the sample    -   (iii) removal of the supernatant from the pellet    -   (iv) re-suspending the pellet in a high pH reagent and        incubating for no more than 8 minutes in order to inhibit the        non-micro-organism source of nucleic acid modifying activity        (whilst not affecting the nucleic acid modifying activity of the        micro-organism in the sample)    -   (v) adding a pH lowering reagent in order to stop the incubation        at high pH    -   (vi) a second centrifugation of the sample to form a pellet        containing micro-organisms if present in the sample    -   (vi) removal of the supernatant from the pellet    -   (vii) lysing any micro-organisms in the pellet    -   (viii) contacting the sample with a nucleic acid molecule which        acts as a substrate for nucleic acid modifying activity of the        micro-organism in the sample,    -   (ix) incubating the thus contacted sample under conditions        suitable for nucleic acid modifying activity; and    -   (x) specifically determining the absence or presence of a        modified nucleic acid molecule resulting from the action of the        nucleic acid modifying activity on the substrate nucleic acid        molecule to indicate the absence or presence of the        micro-organism.

17. The method of clause 16 wherein the high pH reagent comprises NaOHor Na2CO3.

18. The method of clause 16 or 17 wherein the concentration of the highpH reagent is around 5 mM or greater.

19. The method of any one of clauses 16 to 18 wherein the pH loweringreagent comprises a buffer or an acid.

20. The method of clause 19 wherein the buffer comprises a Tris-HClbuffer (pH 7.2 or 8)

21. The method of any one of clauses 16 to 20 wherein step (a)(iii) or(b)(iv) is performed at a temperature between 15 and 30 degrees Celsius.

22. The method of any one of clauses 16 to 21 wherein step (a)(iii) or(b)(iv) is performed at room temperature.

23. The method of any one of clauses 16 to 22 wherein the method isperformed at a temperature between 15 and 30 degrees Celsius.

24. The method of any one of clauses 16 to 23 wherein the method isperformed at room temperature.

25. A method of detecting the absence or presence of a micro-organism ina (liquid) sample, the sample potentially containing anon-micro-organism source of nuclease activity comprising:

-   -   (i) incubation of the sample with a reagent that lyses        non-micro-organisms if present in the sample but does not lyse        micro-organisms in the sample    -   (ii) separation of the lysed cell material from the intact        microorganisms (if any) in the sample and/or inactivation of the        lysed cell material    -   (iii) lysing any microorganisms following the separation and/or        inactivation    -   (iv) contacting the sample with a nucleic acid molecule which        acts as a substrate for nucleic acid modifying activity of the        micro-organism in the sample together with an internal positive        control (IPC) nucleic acid molecule,    -   (v) incubating the thus contacted sample under conditions        suitable for nucleic acid modifying activity; and    -   (vi) specifically determining the absence or presence of a        modified nucleic acid molecule resulting from the action of the        nucleic acid modifying activity on the substrate nucleic acid        molecule to indicate the absence or presence of the        micro-organism, characterised in that the IPC nucleic acid        molecule is modified so as to protect it from nuclease activity

or

-   -   (a) centrifugation of the sample to form a pellet containing        micro-organisms if present in the sample    -   (b) removal of the supernatant from the pellet    -   (c) lysing any micro-organisms in the pellet    -   (d) contacting the sample with a nucleic acid molecule which        acts as a substrate for nucleic acid modifying activity of the        micro-organism in the sample together with an internal positive        control (IPC) nucleic acid molecule,    -   (e) incubating the thus contacted sample under conditions        suitable for nucleic acid modifying activity; and    -   (f) specifically determining the absence or presence of a        modified nucleic acid molecule resulting from the action of the        nucleic acid modifying activity on the substrate nucleic acid        molecule to indicate the absence or presence of the        micro-organism, characterised in that the IPC nucleic acid        molecule is modified so as to protect is from nuclease activity.

26. The method of clause 25 wherein the nucleic acid molecule ismodified so as to protect it from nuclease activity.

27. The method of clause 25 or 26 wherein the modification is selectedfrom incorporation of methylation, protection of the 3′ and/or 5′ ends,incorporation of synthetic nucleotides.

28. The method of clause 27 wherein the synthetic nucleotides comprisephosphorothioate nucleotides and/or locked nucleic acid nucleotides.

29. A method of detecting the absence or presence of a micro-organism ina (liquid) sample, the sample potentially containing anon-micro-organism source of nuclease activity comprising:

-   -   (i) incubation of the sample with a reagent that lyses        non-micro-organisms if present in the sample but does not lyse        micro-organisms in the sample    -   (ii) separation of the lysed cell material from the intact        microorganisms (if any) in the sample and/or inactivation of the        lysed cell material    -   (iii) lysing any microorganisms following the separation and/or        inactivation    -   (iv) contacting the sample with a nucleic acid molecule which        acts as a substrate for nucleic acid modifying activity of the        micro-organism in the sample together with an internal positive        control (IPC) nucleic acid molecule,    -   (v) incubating the thus contacted sample under conditions        suitable for nucleic acid modifying activity; and    -   (vi) specifically determining the absence or presence of a        modified nucleic acid molecule resulting from the action of the        nucleic acid modifying activity on the substrate nucleic acid        molecule to indicate the absence or presence of the        micro-organism, characterised in that the IPC nucleic acid        molecule is susceptible to nuclease activity and is used to        identify contaminating nuclease activity in the pellet.

or

-   -   (a) centrifugation of the sample to form a pellet containing        micro-organisms if present in the sample    -   (b) removal of the supernatant from the pellet    -   (c) lysing any micro-organisms in the pellet    -   (d) contacting the sample with a nucleic acid molecule which        acts as a substrate for nucleic acid modifying activity of the        micro-organism in the sample together with an internal positive        control (IPC) nucleic acid molecule,    -   (e) incubating the thus contacted sample under conditions        suitable for nucleic acid modifying activity; and    -   (f) specifically determining the absence or presence of a        modified nucleic acid molecule resulting from the action of the        nucleic acid modifying activity on the substrate nucleic acid        molecule to indicate the absence or presence of the        micro-organism, characterised in that the IPC nucleic acid        molecule is susceptible to nuclease activity and is used to        identify contaminating nuclease activity in the pellet.

30. The method of any one of clauses 25 to 29 wherein steps (iii) and(iv) or (c) and (d) respectively are performed together.

31. The method of clause 30 wherein the nucleic acid molecule is addedto the sample together with a lysis reagent.

32. The method of any one of clauses 1 to 31 wherein specificallydetermining the absence or presence of the modified nucleic acidmolecule comprises a nucleic acid amplification step.

33. The method of clause 32 wherein the nucleic acid molecule is atleast partially double stranded and comprises uracil residues in thecomplementary strand and the step of specifically determining theabsence or presence of the modified nucleic acid molecule comprisesadding Uracil DNA Glycosylase (UDG) to the sample in order to degradethe uracil residues in the complementary strand.

34. The method of any one of clauses 25 to 33 wherein the IPC nucleicacid molecule comprises identical primer binding sites to the nucleicacid molecule such that there is competition for primer binding in step(f).

35. The method of any one of clauses 25 to 34 wherein a nucleic acidprobe is added in step (f) which binds to a target probe sequence withinthe (sense strand of the) nucleic acid molecule.

36. The method of any one of clauses 25 to 35 wherein a further nucleicacid probe is added in step (f) which binds to a target probe sequencewithin the IPC nucleic acid molecule.

37. The method of clause 36 wherein the nucleic acid probe does not bindto the IPC nucleic acid molecule and the further nucleic acid probe doesnot bind to the nucleic acid molecule.

38. The method of any one of clauses 35 to 37 wherein the nucleic acidprobe is labelled.

39. The method of any one of clauses 35 to 38 wherein the furthernucleic acid probe is labelled.

40. The method of clause 38 or 39 wherein the nucleic acid probe andfurther nucleic acid probe are differently labelled

41. The method of clause 33 wherein the complementary strand of thenucleic acid molecule comprises a modification at the 3′ end to preventextension.

42. The method of clause 41 wherein the modification comprisesincorporation of a non-extendible nucleotide

43. The method of clause 42 wherein the non-extendible nucleotide is adideoxy nucleotide triphosphate (ddNTP).

44. The method of clause 43 wherein the ddNTP is dideoxyCytidine.

45. The method of any one of clauses 29 to 44 wherein the nucleic acidmolecule is modified so as to protect it from nuclease activity.

46. The method of clause 45 wherein the modification is selected fromincorporation of methylation, protection of the 3′ and/or 5′ ends,incorporation of synthetic nucleotides.

47. The method of clause 46 wherein the synthetic nucleotides comprisephosphorothioate nucleotides and/or locked nucleic acid nucleotides.

48. A method of detecting the absence or presence of a microorganism ina sample, the sample containing a non-microorganism source of nucleicacid modifying activity comprising:

-   -   (i) incubation of the sample with a reagent that lyses        non-microorganisms if present in the sample but does not lyse        microorganisms in the sample    -   (ii) optionally separation of the lysed cell material from the        intact microorganisms (if any) in the sample    -   (iii) contacting the (separated) intact microorganisms (if any)        in the sample with a high pH reagent and incubating for no more        than 5 minutes in order to inhibit the non-micro-organism source        of nucleic acid modifying activity (whilst not affecting the        nucleic acid modifying activity of the micro-organism in the        sample)    -   (iv) adding a pH lowering reagent in order to stop the        incubation at high pH    -   (v) separation of the microorganisms if present in the sample        from the pH modifying reagents    -   (vi) lysis of any separated microorganisms    -   (vii) contacting the sample with a nucleic acid molecule which        acts as a substrate for nucleic acid modifying activity of the        micro-organism in the sample,    -   (viii) incubating the thus contacted sample under conditions        suitable for nucleic acid modifying activity; and    -   (ix) specifically determining the absence or presence of a        modified nucleic acid molecule resulting from the action of the        nucleic acid modifying activity on the substrate nucleic acid        molecule to indicate the absence or presence of the        micro-organism, wherein the nucleic acid molecule is modified so        as to protect it from nuclease activity.

or

-   -   (a) incubation of the sample with a reagent that lyses        non-micro-organisms if present in the sample but does not lyse        micro-organisms in the sample    -   (b) centrifugation of the sample to form a pellet containing        micro-organisms if present in the sample    -   (c) removal of the supernatant from the pellet    -   (d) re-suspending the pellet in a high pH reagent and incubating        for no more than 8 minutes in order to inhibit the        non-micro-organism source of nucleic acid modifying activity        (whilst not affecting the nucleic acid modifying activity of the        micro-organism in the sample)    -   (e) adding a pH lowering reagent in order to stop the incubation        at high pH    -   (f) a second centrifugation of the sample to form a pellet        containing micro-organisms if present in the sample    -   (g) removal of the supernatant from the pellet    -   (h) lysing any micro-organisms in the pellet    -   (i) contacting the sample with a nucleic acid molecule which        acts as a substrate for nucleic acid modifying activity of the        micro-organism in the sample,    -   (j) incubating the thus contacted sample under conditions        suitable for nucleic acid modifying activity; and    -   (k) specifically determining the absence or presence of a        modified nucleic acid molecule resulting from the action of the        nucleic acid modifying activity on the substrate nucleic acid        molecule to indicate the absence or presence of the        micro-organism, wherein the nucleic acid molecule is modified so        as to protect it from nuclease activity.

49. The method of clause 48 wherein the modification is selected fromincorporation of methylation, protection of the 3′ and/or 5′ ends,incorporation of synthetic nucleotides.

50. The method of clause 49 wherein the synthetic nucleotides comprisephosphorothioate nucleotides and/or locked nucleic acid nucleotides.

51. The method of any one of clauses 48 to 50 further characterised inthat the nucleic acid molecule is added to the sample at a concentrationof at least 2 nM and less than 50 nM.

52. The method of any one of clauses 48 to 51 wherein step (vii) or (i)respectively comprises adding to the sample deoxyribonucleotidetriphosphates at a concentration of at least 100 μM.

53. The method of any one of clauses 48 to 52 wherein the high pHreagent comprises NaOH or Na2CO3.

54. The method of any one of clauses 48 to 53 wherein the concentrationof the high pH reagent is around 5 mM or greater.

55. The method of any one of clauses 48 to 54 wherein the pH loweringreagent comprises a buffer or an acid.

56. The method of clause 55 wherein the buffer comprises a Tris-HClbuffer (pH 7.2 or 8)

57. The method of any one of clauses 48 to 56 wherein step (iv) or (d)respectively is performed at a temperature between 15 and 30 degreescelcius.

58. The method of any one of clauses 48 to 57 wherein step (iv) or (d)respectively is performed at room temperature.

59. The method of any one of clauses 48 to 58 wherein the method isperformed at a temperature between 15 and 30 degrees celcius.

60. The method of any one of clauses 48 to 59 wherein the method isperformed at room temperature.

61. The method of any one of clauses 48 to 60 wherein step (vi) or (i)respectively comprises contacting the sample with a nucleic acidmolecule which acts as a substrate for nucleic acid modifying activityof the micro-organism in the sample together with an internal positivecontrol (IPC) nucleic acid molecule, wherein the IPC nucleic acidmolecule is susceptible to nuclease activity and is used to identifycontaminating nuclease activity in the pellet.

62. The method of any one of clauses 48 to 60 wherein step (vi) or (i)respectively comprises contacting the sample with a nucleic acidmolecule which acts as a substrate for nucleic acid modifying activityof the micro-organism in the sample together with an internal positivecontrol (IPC) nucleic acid molecule, wherein the IPC nucleic acidmolecule is modified so as to protect it from nuclease activity.

63. The method of clause 62 wherein the modification is selected fromincorporation of methylation, protection of the 3′ and/or 5′ ends,incorporation of synthetic nucleotides.

64. The method of clause 63 wherein the synthetic nucleotides comprisephosphorothioate nucleotides and/or locked nucleic acid nucleotides.

65. The method of any one of clauses 48 to 64 wherein steps (vi) and(vii) or (h) and (i) respectively are performed together

66. The method of clause 65 wherein the nucleic acid molecule is addedto the sample together with a lysis reagent.

67. The method of any one of clauses 48 to 66 wherein step (xi) or (k)respectively comprises a nucleic acid amplification step.

68. The method of any one of clauses 48 to 67 wherein the nucleic acidmolecule is at least partially double stranded and comprises uracilresidues in the complementary strand and step (k) comprises addingUracil DNA Glycosylase (UDG) to the sample in order to degrade theuracil residues in the complementary strand.

69. The method of any one of clauses 64 to 68 wherein the IPC nucleicacid molecule comprises identical primer binding sites to the nucleicacid molecule such that there is competition for primer binding in step(xi) or (k) respectively.

70. The method of any one of clauses 48 to 69 wherein a nucleic acidprobe is added in step (xi) or (k) respectively which binds to a targetprobe sequence within the (sense strand of the) nucleic acid molecule.

71. The method of clause 70 wherein a further nucleic acid probe isadded in step (xi) or (k) respectively which binds to a target probesequence within the IPC nucleic acid molecule.

72. The method of clause 71 wherein the nucleic acid probe does not bindto the IPC nucleic acid molecule and the further nucleic acid probe doesnot bind to the nucleic acid molecule.

73. The method of any one of clauses 70 to 72 wherein the nucleic acidprobe is labelled.

74. The method of any one of clauses 71 to 73 wherein the furthernucleic acid probe is labelled.

75. The method of clause 74 wherein the nucleic acid probe and furthernucleic acid probe are differently labelled.

76. The method of any one of clauses 68 to 75 wherein the complementarystrand of the nucleic acid molecule comprises a modification at the 3′end to prevent extension.

77. The method of clause 76 wherein the modification comprisesincorporation of a non-extendible nucleotide.

78. The method of clause 77 wherein the non-extendible nucleotide is adideoxy nucleotide triphosphate (ddNTP).

79. The method of clause 78 wherein the ddNTP is dideoxyCytidine.

80. The method of any one of clauses 1 to 79 wherein the nucleic acidmodifying activity comprises polymerase activity.

81. Use of the method according to any one of clauses 1 to 80 forscreening for resistance of a micro-organism to an agent directedagainst micro-organism.

82. Use of the method according to any one of clauses 1 to 80 forscreening candidate agents which may be capable of killing or preventinggrowth of one or more micro-organisms.

83. Use of the method according to any one of clauses 1 to 80 fordiagnosing an infection, or a disease associated with the presence of amicro-organism in a subject.

84. Use of the method according to any one of clauses 1 to 80 fordetecting the presence of micro-organism contamination in a plateletcontaining sample.

85. A kit for carrying a method according to any one of clauses 1 to 84comprising:

-   -   (a) at least one nucleic acid molecule which acts as a substrate        for nucleic acid modifying activity of the micro-organism in the        sample, wherein the at least one nucleic acid molecule is at        least partially double stranded and comprises uracil residues in        the complementary strand, characterised in that the nucleic acid        molecule is modified so as to protect it from nuclease activity    -   (b) at least one internal positive control (IPC) nucleic acid        molecule which comprises identical primer binding sites to the        nucleic acid molecule such that there is competition for primer        binding in a nucleic acid amplification reaction containing both        the nucleic acid molecule and the IPC.

86. The kit of clause 85 wherein the kit further comprises a nucleicacid probe which binds to a target probe sequence within the (sensestrand of the) nucleic acid molecule.

87. The kit of clause 85 or 86 wherein the kit further comprises afurther nucleic acid probe which binds to a target probe sequence withinthe IPC nucleic acid molecule.

88. The kit of clause 86 or 87 wherein the nucleic acid probe does notbind to the IPC nucleic acid molecule and the further nucleic acid probedoes not bind to the nucleic acid molecule.

89. The kit of any one of clauses 86 to 88 wherein the nucleic acidprobe is labelled.

90. The kit of any one of clauses 87 to 89 wherein the further nucleicacid probe is labelled.

91. The kit of any one of clauses 87 to 90 wherein the nucleic acidprobe and further nucleic acid probe are differently labelled.

92. The kit of any one of clauses 85 to 91 wherein the complementarystrand of the nucleic acid molecule comprises a modification at the 3′end to prevent extension.

93. The kit of clause 92 wherein the modification comprisesincorporation of a non-extendible nucleotide.

94. The kit of clause 93 wherein the non-extendible nucleotide is adideoxy nucleotide triphosphate (ddNTP).

95. The kit of clause 94 wherein the ddNTP is dideoxyCytidine.

96. The kit of any one of clauses 85 to 95 wherein the IPC is modifiedso as to protect it from nuclease activity.

97. The kit of clause 96 wherein the modification is selected fromincorporation of methylation, protection of the 3′ and/or 5′ ends,incorporation of synthetic nucleotides.

98. The kit of clause 97 wherein the synthetic nucleotides comprisephosphorothioate nucleotides and/or locked nucleic acid nucleotides.

99. The kit of any one of clauses 85 to 98 wherein the kit furthercomprises a high pH reagent.

100. The kit of clause 99 wherein the high pH reagent comprises NaOH orNa2CO3.

101. The kit of clause 99 or 100 wherein the concentration of the highpH reagent is around 5 mM or greater.

102. The kit of any one of clauses 85 to 101 further comprising a pHlowering agent.

103. The kit of clause 102 wherein the pH lowering reagent comprises abuffer or an acid.

104. The kit of clause 103 wherein the buffer comprises a Tris-HClbuffer (pH 7.2 or 8).

DESCRIPTION OF THE FIGURES

FIG. 1. Improved ETGA detection of microorganisms by increasing dNTP andsubstrate concentration. Each chart shows the ct value obtained for thedetection of the ETGA target substrate (FAM channel) in ETGA detectionexperiments for a range of relevant microorganisms. In all cases thenegative blood culture control was >39.9 ct units, the negative reagentnegative controls were >40 ct units and the positive reagent controlswere <20 ct units.

FIG. 2. Detection of IPC molecule in a blood culture sample preparedwith IPC DNA added only in the microbial Lysis mixture (LM) or PCRmastermix (MM) compared to a negative control sample. A quantity of IPCDNA was added to the LM to provide the same ct value as when added tothe MM. Data shows that there is a complete loss in detection of the IPCmolecule (in a 40 cycle PCR reaction) in the negative blood culturesample compared to the negative control (with no blood) when added to LMand not when added to MM.

FIG. 3. Increased background caused by adding IPC to LM instead of MM.The data plotted on the chart shows ct value in the FAM channel(detecting ETGA substrate) versus total viable count (TVC) obtained forblood culture samples using a protocol where the IPC had been added toLM (diamonds) and MM (squares). The amount of IPC added to LM wasequivalent to 50× higher than in MM for each PCR reaction. The measuredbackground was higher when using 50× the normal concentration of IPC inLM compared to using the standard concentration of IPC in MM. Backgroundlevels were measured by an ETGA test procedure with IPC in the MM (bluedashed line) or LM (green dot-dashed line) in blood culture samples thatdid not contain any added bacteria.

FIG. 4. ETGA test background reduction and improved test sensitivity.Graphs show fluorescence detected in the FAM channel in qPCR reactionfrom ETGA tests carried out on a dilution series of C. albicans in bloodculture. The qPCR reaction contained a FAM-labelled probe, capable ofdetecting the modified ETGA substrate, so amplification indicates thepresence of a microorganism. FIG. 4a shows the results of a set of ETGAtests carried out using standard substrate and IPC oligos. FIG. 4b showsthe results obtained from exactly the same samples using PTO substrateand IPC. Note that background is much lower in FIG. 4b , and that it ispossible to detect lower number of yeast cells in the test.

FIG. 5. Improvement of yeast detection by use of PTO oligos. Graph showssensitivity of detection of yeast (C. albicans) in an ETGA test usingstandard oligos compared to an ETGA test using PTO oligos.

FIG. 6. Detection of less robust microorganisms by ETGA. Chart shows howdetection of a delicate strain of H. influenzae is affected by the ETGAtest procedure. Pure culture of S. aureus and H. influenzae (10⁵ cfu)was added to the general test protocol (10 mL) at different stages. Datashows that detection was significantly reduced when microorganisms areadded before the NaOH resuspension step.

FIG. 7. Controlling exposure to NaOH to improve detection of H.influenzae. Graph shows the effect of controlling the amount of timethat a culture sample containing 10⁵ cfu H. influenzae is exposed toNaOH in the ETGA test procedure compared to the standard procedure. Thegeneral protocol for 10 mL was carried out on a suspension of H.influenzae in BacT/ALERT broth without blood; after resuspension in NaOHand incubation for 0, 0.5, 2.5 and 5 min, 1 mL 200 mM Tris-HCl [pH7.2]was added prior to centrifugation.

FIG. 8. Improving the 1 ml ETGA protocol with a pH lowering step. Bloodculture samples containing A) no spike, B) H. influenzae (10⁵ cfu), C)H. influenzae (10⁴ cfu), D) S. aureus (10⁵ cfu), E) S. aureus (10⁴ cfu)were tested with the original 1 ml procedure (based on a resuspension in1 mL NaOH) and a procedure containing a pH-lowering step (resuspensionin 0.75 mL NaOH, 5 min incubation, 0.5 mL Reagent C). The lower ct valueindicates that the microorganisms are detected more strongly.

FIGS. 9A-C. Cognitor Minus results for E. coli spiked blood brothsamples and the positive control (Pol(+)). Data is shown for CognitorMinus samples analysed at time 0, 2 and 20 hours with or without the 95°C. step for (A) experiment 2, (B) experiment 3 and (C) experiment 4.Data for experiment 1 is not shown because samples were only analysed byQPCR at time 0 hours.

FIG. 10. Ct values for E. coli spiked blood broth samples (n=4) plottedagainst log transformed total cfu values. Trend lines are plotted foreach time point (0 hours, 2 hours and 20 hours) within the 95° C. (+) or95° C. (−) data sets. Positive control data is not shown here.

FIGS. 11A-B. Ct values for E. coli spiked blood broth samples andpositive controls (Pol+ve) at time 0, 2 and 20 hours for 95° C. (+) and95° C. (−) samples processed using either (A) unmodified oligonucleotidelysis mix or (B) phosphorothioate oligonucleotide lysis mix. The datashown is from a single experiment (n=1).

FIGS. 12A-E. Cognitor Minus results for (A) E. coli, (B) S. aureus and(C) C. albicans spiked blood broth samples, (D) positive controls (PC)and (E) no spike controls (NSCs). Ct values from three replicateexperiments (n=3) are plotted against sample storage duration. Trendlines are plotted for each sample set: PTO 95° C. (+); PTO 95° C. (−);UMO 95° C. (+); and UMO 95° C. (−). Only UMO LM data are shown for NSCsamples because most PTO LM samples produced ‘No Ct’ due to insufficientamplification.

EXPERIMENTAL SECTION

The invention will be understood with respect to the followingnon-limiting examples:

Example 1—ETGA Test Modifications

Methods—General Protocol

For each sample, 1 mL of blood culture (with or without microorganismsadded, with or without blood) was mixed with 0.333 mL Reagent A (5% w/vSaponin, 5% w/v Tween 20, 8.5 g/L sodium chloride) in a 1.5 mLmicrocentrifuge tube and incubated at room temperature for 15 min. Eachsample was centrifuged for 3 min at 7300 g, then the supernatant waspoured away and the rim of the tube was dabbed on clean laboratorytissue paper. Each pellet was then resupended in 0.75 mL of Reagent B (5mM NaOH) and incubated for 5 min, then pH was lowered by adding 0.5 mLof Reagent C (1.32 g/L ammonium sulphate, 0.49 g/L magnesium sulphateheptahydrate, 0.75 g/L potassium chloride, 20 mM Tris-HCl, pH8.0). Afterincubation, samples were centrifuged again and the supernatant removedby pouring away. The remaining pellet was resuspended in 0.5 mL ofReagent C and immediately transferred to a new tube containing a mixtureof glass beads (0.1 mm and 0.5 mm glass beads; supplied by CamBio cat13118-400, and 13116-400 respectively). A further centrifugation wascarried out in order to pellet any suspended cells with the glass beads,and again, the supernatant was removed and discarded.

50 μL of microbial Lysis Mixture containing the ETGA substrate (LM;containing reagents L1, L2, L3 at a ratio of 7:2:1, see Table 1) wasadded to the glass beads and placed in a Disruptor Genie (ScientificIndustries, Inc.) cell disruptor for 6 min at 2800 rpm to lyse microbialcells. After disruption, samples were placed in a 37° C. heating blockand incubated for 20 min, then transferred to another heating block at95° C. and incubated for 5 min. After incubation, samples were cooled toroom temperature whilst the PCR reagents were prepared.

After cooling, 3 μL of sample supernatant was added to 27 μL of PCRmastermix (MM; containing a general Taq polymerase PCR mastermix(Roche—cat 04902343001), primers for the ETGA substrate, internalpositive control—IPC—DNA, FAM-labelled probe for the ETGA substrate,Texas Red labelled probe for the IPC, and (1.2 ul) UDG enzyme(Bioline—cat no BIO-27044)) in a SmartCycler PCR tube (Cepheid). Sampleswere placed in the SmartCycler PCR and subjected to the followingreaction conditions;

1 cycle; 40° C. 10 min, 50° C. 10 min, 95° C. 5 min

40-50 cycles: 95° C. 5 sec, 61° C. 20 sec, 72° C. 20 sec.

Amplification was monitored throughout the reaction in real-time in theTexas Red and FAM excitation/detection channels of the SmartCycler.

TABLE 1 Lysis mixture components L1 Bovine serum albumin   1.5% w/vTriton X100   1.5% v/v Tween 20   1.5% v/v L2 Ammonium sulphate  2.64 g/L Magnesium sulphate   0.98 g/L heptahydrate Potassium chloride  1.5 g/L Tris-HCl, pH 8.0  40 mM dNTP (A,G,C,T) 500 μM L3ETGA substrate   0.001 μM-0.01 μM Tris-HCl, pH 8.5  20 mM KCl  10 mMEDTA  10 μM

The sequences of the PCR reaction components are as follows;

Fam labelled probe (a molecular beacon): (SEQ ID NO: 1)FAM-cgc tgc gac cga ccg ata agc tag aac agg cag cg-BHQ1Texas red labelled probe (a molecular beacon): (SEQ ID NO: 2)TxR-cgc gat cag cag gcc aca cgt taa aga cat cgc g-BHQ2 IPC(SEQ ID NO: 3) gcc gat atc gga caa cgg ccg aac tgg gaa ggc gagatc agc agg cca cac gtt aaa gac aga gag aca acaacg ctg gcc gtt tgt cac cga cgc cta Forward primer (SEQ ID NO: 4)ccg ata tcg gac aac ggc cga act gg Reverse primer (SEQ ID NO: 5)tag gcg tcg gtg aca aac ggc cag c

The substrate components are;

AS (SEQ ID NO: 6) uaggcgucggugacaaacggccagcguuguugucucu-DDC (3′terminal is a dideoxy-C) S1 (SEQ ID NO: 7)gccgatatcggacaacggccgaactgggaaggcgagactgaccgaccgataagctagaacagagagacaacaac

Results and Discussion 1—Increasing Substrate Concentration

The general protocol was modified by increasing the amount of ETGAsubstrate in LM by 10-fold (from 0.001 μM to 0.01 μM) and increasing theamount of dNTP 2-fold (from 50 μM to 100 μM).

The increased quantity of substrate and dNTP enabled improved detectionof C. albicans (FIG. 1a ), E. coli (FIG. 1b ), S. aureus (FIG. 1c ), E.faecalis (FIG. 1d ), P. aeruginosa (FIG. 1e ), or 2 different strains ofH. influenzae (including a delicate clinical strain) (FIG. 1f and FIG.1g ). Data is shown in FIG. 1.

Results and Discussion 2—Improving ETGA Test Sensitivity and LoweringBackground by Modifying Oligo Components

Evidence of Nuclease Activity

Adding the IPC molecule at the same time as the ETGA substrate moleculewas thought to be an improvement on the original protocol. If IPC wasadded in LM, the IPC would be subject to exactly the same testconditions as the ETGA substrate and therefore provide a more accuratetest control. For example, conditions that may negatively impact thesubstrate molecule such as nuclease activity that could digest thesubstrate, would also affect the IPC. If the IPC is added later (in MMfor example) it would not be subject to the same conditions and mayresult in false interpretation of the data. If IPC is added at the sametime as the ETGA substrate the magnitude of the effect of the testconditions on the nucleic acid templates could be measured.

To exemplify this, FIG. 2 shows that test conditions could have anegative impact on the detection of the ETGA substrate; when addingcomparable amounts of IPC DNA in LM rather than in MM, it was found thatthere was indeed a loss in the ability to detect the IPC in bloodculture specimens compared to negative control samples (without blood).The same loss of detection was not seen when using IPC in MM. Loss ofthe IPC when added in LM was attributed to nucleases that may have beenactive during the 37° C. incubation step of the ETGA protocol. Nucleaseswere most likely to have originated from the blood specimen.

Clearly, if the IPC molecule was lost during the test, the ETGAsubstrate molecule could also be lost.

Loss of the ETGA substrate molecule in a positive blood culture samplewould obviously result in reduced detection sensitivity or potentiallylead to a false negative result, but, by adding IPC to LM it would bepossible to determine (and perhaps quantify) suspected nuclease activityand interpret results accordingly. Samples where a drop in IPC quantitywas observed (as seen by a rise in ct value compared to a negativereagent control) could be reported as ‘unresolved’ rather than‘negative’, thus indicating that the sample was subject to nucleaseactivity and may, in fact, be positive.

Adding an IPC molecule to LM could improve the ETGA test. However,depending on how common nuclease activity was found to be in clinicalspecimens, this could raise the overall number of unresolved resultsrendering the test less attractive to potential users due to the highperceived failure rate (and thus explaining why IPC was originally addedto MM rather than LM).

Testing showed that the IPC molecule could be added to the test in theLM and still be detected in the presence of presumed nuclease activityby increasing the concentration 20-50 fold, but high levels ofbackground were detected even in negative blood culture samples (FIG.3). In this case, background may have been caused by the presence ofpartly digested oligonucleotides that interfere in the detection PCRreaction. Obviously, high levels of background may reduce thesensitivity of the test, especially when attempting to detect very lownumbers of bacteria.

Whilst increasing the amount of IPC in LM may be considered a solutionto the problem of loss of target DNA molecules due to nuclease activity,it may only mask the issue and as previously mentioned may contribute tohigher background level. Increasing the amount of IPC molecule insamples where contaminating nuclease activity was low could also reducetest sensitivity by increasing competition for reaction components inthe detection PCR thereby reducing the ability of the test to detect thetarget substrate. A better solution to the putative nuclease problem wasrequired.

Protecting ETGA Substrate and IPC From Nuclease Activity

Based on the assumption that the IPC molecule should be included in LM,and that nuclease activity may be having a detrimental effect on theETGA test, attempt was made to protect the DNA targets (IPC and ETGAsubstrate) from nuclease degradation. DNA can be protected from nucleaseactivity by various means, by modification (e.g. methylation, endmodification) or using non-standard nucleotides during the synthesis ofsynthetic oligonucleotides (e.g. locked nucleic acids, phopsphorothioatenucleotides).

ETGA test has been found to be less sensitive to yeasts than bacteria.The reason for this not known but is likely to be due to a combinationof reason such as, differences in in vitro activity or absolute quantityof the fungal enzymes in cells compared to the bacterial enzymes, orsensitivity to inhibitors.

Demonstration of the Use of PTO Oligos in the ETGA Test

Standard ETGA substrate and IPC oligos were replaced withphosphorothioate oligos (PTO) with the same nucleotide sequence in LM inthe general protocol. PTO-modified ETGA substrate was added to LM at0.01 μM and PTO-modified IPC was added at a sufficient quantity toachieve a ct value of 37-41 in a 50 cycle PCR reaction.

A dilution series of yeast cells (10⁵, 10⁴, 10³ and 0 cfu/ml) in bloodculture was tested with the original ETGA test protocol with standardoligos and with PTO modified oligos. Both tests were run on exactly thesame spiked blood cultures (FIG. 4).

Data showed that the ETGA test with standard oligos yielded results thatincluded high levels of background that may have occluded the detectionof low levels of yeast cells, whereas results obtained with PTO modifiedoligos did not suffer from the same effect. In fact, results showed thatPTO oligos reduced the overall background to undetectable levels on thesame culture specimens, thus allowing the lowering of the thresholdlevel in the qPCR reaction and potentially increasing the sensitivity ofthe ETGA test. Note that when using standard oligos, the threshold levelwas set at 50 units due to the amount of background fluorescencedetected, but, when using the PTO oligos the threshold level could belowered to 10 units, or lower if required. PTO oligos were detectedlater in the PCR reaction than when using standard oligos, but the PTOqPCR reaction could also be run for longer (50 cycles rather than 40)because the level of background was so low. The result of this reductionin background meant that lower levels of microbial load (as low as 10³cfu/ml in this example) could be detected with PTO oligos that wouldhave previously been undetectable when using the standard oligos.

In a further experiment, nuclease resistant PTO versions of thesubstrate molecules (MWG-Eurofins) were used in an ETGA test. A dilutionseries of yeast overnight culture was used to artificially spike a bloodculture (a sample of the blood culture was then spread on SDA to confirmtotal viable counts). Each suspension was then tested with an ETGA testusing standard oligos and an ETGA test using PTO oligos. Sensitivity ofdetection of the yeast was much improved by the use of PTO. Note thatthe PTO results are plotted on a different y-axis to the standard test,due to the differing number of PCR cycles. The dashed lines indicate theCt value of the negative control (blood culture without anymicroorganisms). The data from this experiment show that the PTOsubstrate was shown to improve the detection of yeasts (FIG. 5) by1000-fold, and furthermore, it did not display the effect of raisingbackground levels. The finding that a PTO substrate could be used toincrease the sensitivity of the ETGA test was very significant.

Example 2—Reducing False Negative Test Results in the ETGA Test

General Protocols

For 10 mL Specimens

For each sample, 10 mL of blood culture (with or without microorganismsadded, with or without blood) was mixed with 3.33 mL Reagent A (5% w/vSaponin, 5% w/v Tween 20, 8.5 g/L sodium chloride) in a 15 mL Falcontube and incubated at room temperature for 15 min. Each sample wascentrifuged for 8 min at 3600 g, then the supernatant was poured awayand the rim of the tube was dabbed on clean laboratory tissue paper.Each pellet was then resupended in 5 mL of Reagent B (5 mM NaOH) andincubated for 5 min. After incubation, samples were centrifuged againand the supernatant removed by pouring away. The remaining pellet wasresuspended in 1 mL of Reagent C (1.32 g/L ammonium sulphate, 0.49 g/Lmagnesium sulphate heptahydrate, 0.75 g/L potassium chloride, 20 mMTris-HCl, pH8.0) and immediately transferred to a 1.5 mL microcentrifugetube containing a mixture of glass beads. A further centrifugation for 3min at 7300× g was carried out in order to pellet any suspended cellswith the glass beads, and again, the supernatant was removed anddiscarded.

50 μL of microbial Lysis Mixture (LM; containing ETGA substrate seetable 1 in Example 1 above) was added to the glass beads and placed in aDisruptor Genie cell disruptor for 6 min at 2800 rpm to lyse microbialcells. After disruption, samples were placed in a 37° C. heating blockand incubated for 20 min, then transferred to another heating block at95° C. and incubated for 5 min. After incubation, samples were cooled toroom temperature whilst the PCR reagents were prepared.

After cooling, 3 μL of sample supernatant was added to 27 μL of PCRmastermix (MM; containing a general Taq polymerase PCR mastermix,primers for the ETGA substrate, internal positive control—IPC—DNA,FAM-labelled probe for the ETGA substrate, Texas Red labelled probe forthe IPC, and UDG enzyme) in a SmartCycler PCR tube (Cepheid). Sampleswere placed in the SmartCycler PCR and subjected to the followingreaction conditions;

1 cycle; 40° C. 10 min, 50° C. 10 min, 95° C. 5 min

40-50 cycles: 95° C. 5 sec, 61° C. 20 sec, 72° C. 20 sec.

Amplification was monitored throughout the reaction in real-time in theTexas Red and FAM excitation/detection channels of the SmartCycler.

For 1 mL Specimens

For each sample, 1 mL of blood culture was mixed with 0.333 mL Reagent A(5% w/v Saponin, 5% w/v Tween 20, 8.5 g/L sodium chloride) in a 1.5 mLmicrocentrifuge tube and incubated at room temperature for 15 min. Eachsample was centrifuged for 3 min at 7300 g, then the supernatant waspoured away and the rim of the tube was dabbed on clean laboratorytissue paper. Each pellet was then resupended in 1 mL of Reagent B (5 mMNaOH) and incubated for 5 min. After incubation, samples werecentrifuged again and the supernatant removed by pouring away. Theremaining pellet was resuspended in 0.5 mL of Reagent C (1.32 g/Lammonium sulphate, 0.49 g/L magnesium sulphate heptahydrate, 0.75 g/Lpotassium chloride, 20 mM Tris-HCl, pH8.0) and immediately transferredto a new tube containing a mixture of glass beads. A furthercentrifugation was carried out in order to pellet any suspended cellswith the glass beads, and again, the supernatant was removed anddiscarded.

Microbial lysis and PCR detection was then carried out as previouslydescribed for the 10 mL protocol.

PCR reaction components and substrates are per Example 1 above.

BACKGROUND

A single clinical microbial isolate, identified as Haemophilusinfluenzae gave a false negative result in the ETGA test during aclinical performance evaluation. The microorganism was detected bystandard automated blood culture in Biomerieux Bact/ALERT blood culturemedia.

When a spike of cultured microbial cells was added to different stagesof the ETGA test it was found that the H. influenzae strain was onlydetected when added after the NaOH wash step (Reagent B). Other, morerobust bacterial species were found to be detectable when added to thetest from the start (see FIG. 6). Results indicated that the strain ofH. influenzae was particularly sensitive to the NaOH washing step (or acombination of the steps up to and including the NaOH washing step).

This result was not typical of all strains of H. influenzae and, todate, has only been associated with this strain. The isolate was used asa model ‘weak’ organism to develop a new ETGA procedure that was betterat detecting less robust microorganisms.

Incubation in NaOH is an essential step in the ETGA protocol that mustbe carried out in order to inactivate free polymerases, reducecontaminants and reduce background. Attempts were therefore made toreduce the damaging effect of NaOH without detrimentally affecting thetest results. The concentration of NaOH could not be lowered because itdid not remove sufficient contaminating material, which leads to failureof the test.

Results—Reducing Sample Exposure to NaOH

In the general 10 mL protocol, note that the time taken to centrifugethe sample increases the total time that the sample is exposed to NaOHby 8 min. Shortening the length of time could be achieved and controlledby neutralisation of the alkali, or, at least lowering the pH of thesample after an optimal period of incubation time by adding 1 mL of 200mM Tris-HCl buffer, pH 7.2 to the NaOH.

The general protocol for 10 mL specimens was carried out on a suspensionof H. influenzae in culture media drawn from a BacT/ALERT SA bottle (noblood was added). Results demonstrate that neutralisation (orsignificant lowering the pH) of the NaOH lead to lower ct values (fromidentical samples) and therefore improved sensitivity of detection (seeFIG. 7).

Data also suggested that the shorter incubation time would be better,but again, short incubation times did not allow sufficient removal ofcontaminants to enable reliable PCR amplification, leading to reactionfailure or high background levels and false positive results.

Lowering of the pH of the sample after NaOH treatment could potentiallybe achieved by adding any suitable buffer or acid. The preferred methodof lowering the pH would be to use Reagent C (a Tris-HCl buffer, pH 8)because the reagent is already used in the test.

Results—Preferred Embodiment, 1 mL

Detection of both H. influenzae and S. aureus could be improved in thegeneral protocol for 1 mL specimens by replacing the original NaOH stepwith the more complex steps consisting of resuspension in 0.75 mL NaOH,incubation at room temperature for 5 min then adding 0.5 mL Reagent C tolower the pH. Results, summarised in FIG. 8, showed that the ct valuesfrom the ETGA tests on all microorganism-containing samples were lowerwhen using the pH-lowering protocol compared to the original 1 mLprotocol, demonstrating that the pH lowering protocol improveddetection.

Example 3—Analysis of the Importance of the 95° C. and the Use of a PTOSubstrate Purpose

The purpose of the work outlined in this example was to assess theeffect of the 95° C. step on the performance of the Cognitor Minus test.The Cognitor Minus test was carried out using bacteria-spiked bloodbroth samples with or without the 95° C. step to compare the followingcharacteristics:

-   -   The Ct values obtained    -   The stability of ETGA template DNA as measured by QPCR at        different time-points following completion of sample preparation    -   The Ct values obtained and stability of ETGA template DNA when        lysis mix (LM) contains unmodified oligonucleotide (UMO) ETGA        substrate as opposed to the phosphorothioate modified        oligonucleotide (PTO) ETGA substrate.

INTRODUCTION

The earlier steps in the Cognitor Minus test aim to lyse blood cells andwash away blood-derived proteins such as DNA polymerases, which willproduce non-microorganism derived ETGA template DNA, and nucleaseenzymes which may digest microorganism-derived ETGA template DNA. Anyresulting intact microorganisms are then lysed by the addition of lysismix (LM) and bead milling. Following microorganism lysis and the ETGAreaction, samples contain a mixture of microorganism proteins, LMcomponents, newly synthesised ETGA template DNA and residual blood cellproteins. The 95° C. step is intended to denature all proteins in orderto protect the ETGA template DNA and internal process control (IPC) DNAfrom nuclease digestion so that it can be successfully detected by QPCR.

To assess the importance of the 95° C. step, the Cognitor Minus test wascarried out using bacteria-spiked blood cultures with or without the 95°C. step. The samples were analysed by QPCR at three time points:immediately after sample preparation; after 2 hours at room temperature;and after a further 18 hours at 4° C. The aim was to compare the Ctvalues obtained and the consistency of Ct values across the three timepoints as an indicator of ETGA template DNA stability. An additionalexperiment was performed using LM containing UMO ETGA substrate to testwhether the stability of the resulting ETGA template DNA differs fromthat of LM containing PTO ETGA substrate.

Materials and Methods

Reagents Used

Reagent A—5% (w/v) Saponin, 5% (v/v) Tween 20 and 146 mM Sodium chloride

Reagent B—5 mM Sodium hydroxide.

Reagent C—10 mM Ammonium sulphate, 2 mM Magnesium sulphate heptahydrate,10 mM Potassium chloride and 20 mM Tris-HCl [pH 8.0].

Lysis Mix (LM) comprised of L1, L2, L3, dNTPs, PTO-IPC stock:

-   -   L1 (252 mL in 360 mL LM)—1.46% (w/v) BSA, 0.15% (v/v) Triton        X100 and 0.15% (v/v) Tween 20;    -   L2 (36 mL in 360 mL LM)—100 mM Ammonium sulphate, 20 mM        Magnesium sulphate heptahydrate, 100 mM Potassium chloride and        200 mM Tris-HCl [pH 8.0];    -   L3 (36 mL in 360 mL LM)—0.1 μM PTO-AS oligo, 0.1 μM PTO-S1        oligo, 20 mM Tris-HCl [pH 8.5], 10 mM Potassium chloride and 10        μM EDTA;    -   10 mM dNTPs (3.6 mL in 360 mL LM)    -   PTO-IPC stock (˜180 μL in 360 mL LM) *Note: variable        concentration    -   H₂O (˜32.22 mL in 360 mL LM)

Method 1: the Cognitor Minus Test on E. coli Spiked Blood Broth with andwithout the 95° C. Step

Escherichia coli (ATCC® 25922™) was grown in nutrient broth for 18 hoursat 37° C. BacT/ALERT SA blood broth (sheep blood) was inoculated toapproximately 1×10⁷ cfu/mL, 1×10⁶ cfu/mL, 1×10⁵ cfu/mL, 1×10⁴ cfu/mL,and 1×10³ cfu/mL with E. coli. Two sets of 1 ml samples were preparedfor testing with or without the 95° C. step. Total viable count (TVC)plates were prepared to confirm cfu/mL values. Two sets of blood brothonly ‘no spike’ controls (NSCs), positive controls (broth only plus DNApolymerase) and negative controls (broth only) were also prepared givinga total of 16 samples (see Table 2).

To each 1 mL sample, 330 μL Reagent A was added and mixed by five tubeinversions. Samples were incubated for 15 minutes at room temperature(approximately 19° C.) and then centrifuged for 3 minutes at 7300 RCF.Following centrifugation, supernatants were decanted into a clinicalwaste receptacle and the open tubes blotted on sterile tissue paper.Each pellet was resuspended in 750 μL Reagent B by tip mixing andincubated for 5 minutes at room temperature. Next, 500 μL Reagent C wasadded to each sample and mixed by three tube inversions. Samples werecentrifuged for 3 minutes at 7300 RCF. The resulting supernatants weredecanted into a clinical waste receptacle and the open tubes blotted onsterile tissue paper. Each pellet was resuspended in 500 μL Reagent C bytip mixing, transferred to a beadmill tube containing glass beads (0.1mm and 0.5 mm glass beads), and centrifuged for 3 minutes at 7300 RCF.Following centrifugation, supernatants were transferred to waste bypipette. 50 μL LM was added to each sample and an additional 10 μL ofDNA Polymerase solution was added to the positive control samples.Samples were then placed into a Disruptor Genie and run for 6 min at2800 rpm. After bead milling, samples were transferred to a heat blockset at 37° C. and incubated for 20 minutes.

Following the 37° C. microorganism lysis (ETGA) step, the 95° C. (−)samples (samples 9-16) were progressed immediately to QPCR setup, whilstthe 95° C. (+) samples (samples 1-8) were incubated at 95° C. for 5minutes prior to QPCR setup. Both sets of samples were analysed by QPCRimmediately. The same samples were analysed by QPCR again after 2 hoursat room temperature (approximately 19° C.), and again after a further 18hours at 4° C. This experiment was replicated four times to allowstatistical analysis of the results.

TABLE 2 Test Samples 95° C. (+) Samples 1. E. coli 1 × 10⁷ cfu/mL 2. E.coli 1 × 10⁶ cfu/mL 3. E. coli 1 × 10⁵ cfu/mL 4. E. coli 1 × 10⁴ cfu/mL5. E. coli 1 × 10³ cfu/mL 6. Blood broth only (NSC) 7. Positive control(Pol + ve) 8. Negative control (Pol − ve) 95° C. (−) Samples 9. E. coli1 × 10⁷ cfu/mL 10. E. coli 1 × 10⁶ cfu/mL 11. E. coli 1 × 10⁵ cfu/mL 12.E. coli 1 × 10⁴ cfu/mL 13. E. coli 1 × 10³ cfu/mL 14. Blood broth only(NSC) 15. Positive control (Pol + ve) 16. Broth only (Pol − ve) NSC: Nospike control

Method 2: PTO ETGA Substrate Vs UMO ETGA Substrate

BacT/ALERT blood broth SA was inoculated to approximately 1×10⁷ cfu/mLand 1×10⁴ cfu/mL with E. coli. Four sets of 1 ml samples were preparedto compare Cognitor Minus test results with and without the use of PTOs,with and without the 95° C. step. TVC plates were prepared to confirmcfu/mL values. NSCs and positive controls were also prepared (see Table3). All samples were processed according to the general protocoldescribed above in “Method 1”. Following the 37° C. microorganism lysis(ETGA) step, the 95° C. (−) samples (samples 9-16) were progressedimmediately to QPCR setup, whilst the 95° C. (+) samples (samples 1-8)were incubated at 95° C. for 5 minutes before QPCR setup. Both sets ofsamples were analysed by QPCR immediately. The same samples wereanalysed by QPCR after 2 hours at room temperature, and again followinga further 18 hours at 4° C.

TABLE 3 Test Samples 95° C. (+) Samples 1. E. coli 1 × 10⁷ cfu/mL: UMOLM 2. E. coli 1 × 10⁷ cfu/mL: PTO LM 3. E. coli 1 × 10⁴ cfu/mL: UMO LM4. E. coli 1 × 10⁴ cfu/mL: PTO LM 5. Blood broth only (NSC): UMO LM 6.Blood broth only (NSC): PTO LM 7. Positive control (Pol + ve): UMO LM 8.Positive control (Pol + ve): PTO LM 95° C. (−) Samples 9. E. coli 1 ×10⁷ cfu/mL: UMO LM 10. E. coli 1 × 10⁷ cfu/mL: PTO LM 11. E. coli 1 ×10⁴ cfu/mL: UMO LM 12. E. coli 1 × 10⁴ cfu/mL: PTO LM 13. Blood brothonly (NSC): UMO LM 14. Blood broth only (NSC): PTO LM 15. Positivecontrol (Pol + ve): UMO LM 16. Positive control (Pol + ve): PTO LM NSC:no spike control, UMO: unmodified oligonucleotide, PTO: phosphorothioateoligonucleotide, LM: lysis mix

Results and Discussion

Results 1: Removal of the 95° C. Step Improves ETGA QPCR Signal withouta Reduction in Signal Over Time

The results for samples processed with or without the 95° C. step attime 0, 2 and 20 hours following sample preparation (n=3) are shown inFIGS. 9A-C. Within individual experiments, removal of the 95° C. stepresulted in reduced Ct values (increased ETGA signal) for all E. colispiked blood broth dilutions and positive controls (FIGS. 9A-C). The Ctvalues obtained for the same samples at time 0, 2 and 20 hours arehighly consistent across the three replicate experiments with maximumΔCt values ranging from 0.07 Ct units to 1.07 Ct units (average maximumΔCt value of 0.30 Ct units) for 95° C. (+) samples and maximum ΔCtvalues ranging from 0.03 Ct units to 0.58 Ct units (average maximum ΔCtvalue of 0.38 Ct units) for 95° C. (−) samples. All NSCs and negativecontrols yielded Ct values greater than 40 or had no QPCR amplificationat all (data not shown). FIG. 10 shows all of the data for E. colispiked blood broth samples plotted together and several trends areapparent. Firstly, there is a clear difference between the Ct valuesobtained for 95° C. (+) samples compared to 95° C. (−) samples, withapproximately a 1.0 Ct unit reduction in Ct values for 95° C. (−)samples. Secondly, there is very little difference between the Ct valuesobtained at different time points for both 95° C. (+) and 95° C. (−)samples. However, there is a small reduction in Ct value as storage timeincreases when the 95° C. step is removed, which is apparent from thetrend lines in FIG. 10 and is more pronounced in experiment 3 (FIG. 9B).

Linear modelling was performed (using R) to determine whether there arestatistically significant differences between the Ct values obtained forthe E. coli spiked blood broth dilution series with or without the 95°C. step and at different time points. Table 4 shows the p-valuesobtained for different comparisons. Comparison of Ct values for 95° C.(+) samples with 95° C. (−) samples using data from all four experimentsproduced highly significant p-values (p<0.001) regardless of whetherdata from individual time points or data from all time points wereincluded in the analysis. Comparison of Ct values for different timepoints within 95° C. (+) or 95° C. (−) datasets using data from all fourexperiments produced non-significant p-values (p>0.05). Time pointcomparisons within the same experiment produced non-significant p-values(p>0.05) for all datasets apart from the 95° C. (−) dataset inexperiment 3 (T3), which had a p-value of 0.016. This significantp-value is likely to be due to the more pronounced reduction in Ct valuewith time that was observed in this particular experiment.

TABLE 4 Linear Models (using R) to compare Ct value standard curves forthe E. coli spiked blood culture dilution series at different timepoints and with or without the 95° C. step. Data analysed ComparisonP-value Significance All data 95° C. (+) vs   <2 × 10⁻¹⁶ *** 95° C. (−)Time 0 H: T1-T4 data 95° C. (+) vs 4.82 × 10⁻⁸ *** 95° C. (−) Time 2 H:T2-T4 data 95° C. (+) vs 1.31 × 10⁻⁸ *** 95° C. (−) Time 20 H: T2-T4data 95° C. (+) vs  1.47 × 10⁻¹⁰ *** 95° C. (−) 95° C. (+): T1-T4 dataTime 0.730 — 95° C. (−): T1-T4 data Time 0.094 — 95° C. (+): T2 dataonly Time 0.896 — 95° C. (−): T2 data only Time 0.657 — 95° C. (+): T3data only Time 0.956 — 95° C. (−): T3 data only Time 0.016 * 95° C. (+):T4 data only Time 0.769 — 95° C. (−): T4 data only Time 0.061 —Significance codes: * p < 0.05, ** p < 0.01, *** p < 0.001. —: notsignificant. T: experiment.

Results 2: Cognitor Minus Results are More Consistent Across DifferentTime Points when Using PTO LM Rather than UMO LM

FIGS. 11A-B shows the Ct values obtained for E. coli spiked blood brothsamples (1×10⁷ cfu/mL and 1×10⁴ cfu/mL) and positive controls processedusing either UMO LM (FIG. 11A) or PTO LM (FIG. 11B) with and without the95° C. step. The data shown here is from a single experiment. The Ctvalues for NSCs using UMO LM were all at least 5 Ct units higher thanthe Ct values obtained for 1×10³ cfu/mL E. coli spiked blood brothsamples, whilst the PTO LM NSC Ct values were all greater than 42.0 Ctunits or had no QPCR amplification at all (data not shown). The resultsdemonstrate that the Ct values for UMO LM samples are on average 10.2 Ctunits lower than the Ct values for PTO LM samples. There is also agreater reduction in Ct value for UMO LM samples than there is for PTOLM samples when the 95° C. step is removed. Most importantly, inrelation to ETGA template DNA stability, the Ct values obtained for UMOLM samples are notably less consistent across the different time pointsthan the Ct values for PTO LM samples. The Ct values for E. coli spikedblood broth samples in the UMO LM 95° C. (+) dataset increased byapproximately 1.0 Ct unit from 2 hours to 20 hours, whilst thecorresponding positive control showed a 0.24 Ct unit reduction in Ctvalue. This indicates that nuclease digestion of ETGA template DNA maybe occurring in samples when bacteria and/or host blood cells arepresent despite the protein denaturing effect of the 95° C. step, butnot in the positive control were only DNA polymerase enzyme is added.When the 95° C. step is removed, the Ct values for UMO LM samplesdecrease with increased sample storage time. This indicates that in theabsence of protein denaturation, continued ETGA template generation mayout compete any increase in nuclease digestion, hence resulting inincreased ETGA QPCR signal. The PTO LM data (FIG. 11B) demonstratehighly consistent Ct values across all time points for both 95° C. (+)and 95° C. (−) samples. These data indicate that ETGA template DNAformed from PTO substrate DNA is more resistant to change by eithernuclease degradation and/or additional ETGA template DNA generationfollowing the 37° C. ETGA reaction.

SUMMARY

The data shown here demonstrate that ETGA template DNA detection isimproved when the 95° C. step is removed. Furthermore, ETGA QPCR signaldoes not deteriorate with increased sample storage time in the absenceof the 95° C. step. Comparison of PTO LM with UMO LM indicates that thehigh stability of ETGA template DNA is dependant of the use of PTO ETGAsubstrate DNA. All of the data shown here support removal of the 95° C.step from the Cognitor Minus test. It is worth noting that the increasedsensitivity of the test may increase the chance of detecting backgroundsignal (blood-derived ETGA signal), however, optimisation of otherfactors such as blood lysis/wash performance and interpretation of QPCRresults should eliminate the impact of this.

Example 4—Analysis of the Importance of the 95° C. and the Use of a PTOSubstrate: Primary Panel Microorganisms

Purpose

The purpose of the work presented in this report was to:

-   -   1. Compare Cognitor Minus results with and without the 95° C.        step for all primary panel microorganisms (E. coli; S. aureus;        and C. albicans)    -   2. Confirm the findings of Example 3 with regard to comparison        between phosphorothioate modified oligonucleotide (PTO) LM and        unmodified oligonucleotide (UMO) LM    -   3. Test the effect of extended sample storage duration (up to 72        hours) on QPCR results for each primary panel microorganism        using PTO LM and UMO LM

INTRODUCTION

The earlier steps in the Cognitor Minus test aim to lyse blood cells andwash away blood-derived proteins such as DNA polymerases, which willproduce non-microorganism derived ETGA template DNA, and nucleaseenzymes which may digest microorganism-derived ETGA template DNA. Thisprocess should not harm any microorganisms that are present in the bloodsample. Isolated intact microorganisms are then lysed by the addition oflysis mix (LM) and bead milling. After microorganism lysis and the ETGAreaction, samples contain a mixture of microorganism proteins, LMcomponents, newly synthesised ETGA template DNA and residual blood cellproteins. In the current Cognitor Minus test protocol, the 95° C. stepis intended to denature all proteins in order to protect ETGA templateDNA and internal process control (IPC) DNA from nuclease digestion sothat it can be successfully detected by QPCR. The 95° C. step alsoinactivates DNA polymerases, thereby quenching the ETGA reaction.However, since incorporating the 95° C. step into the protocol, the UMOsused to form the ETGA substrate (and IPC) in the LM have been replacedwith PTOs which are nuclease resistant. Whilst the ETGA extension strandthat forms the ETGA template is constructed from standard dNTPs, the PTOsubstrate DNA that it is annealed to may confer protection againstnuclease digestion. Due to the benefits of removing the 95° C. step,such as protocol simplification and a reduction in the time required torun the test, it was deemed important to re-evaluate the necessity ofthe 95° C. step.

To assess the importance of the 95° C. step, the Cognitor Minus test wascarried out using microorganism-spiked blood broth with or without the95° C. step. Samples were analysed by QPCR at five time points:immediately after sample preparation; after 2 hours stored at roomtemperature (approximately 19° C.); and after 24 hours, 48 hours and 72hours stored at 4° C. The Ct values obtained and consistency of Ctvalues across the five time points were used to assess the effect of the95° C. step on Cognitor Minus test performance and sample stability foreach of the primary panel microorganisms using either PTO LM or UMO LM.

Materials and Methods

For details of reagents, see Example 3.

Escherichia coli (ATCC® 25922™), Staphylococcus aureus (ATCC® 25923™)and Candida albicans (ATCC® 10231™) were grown in liquid media (E. coliand S. aureus in nutrient broth; and C. albicans in Sabouraud media) forapproximately 18 hours at 37° C. BacT/ALERT SA blood broth (sheep blood;see Table 6) was inoculated with E. coli, S. aureus and C. albicans toapproximately 1×10⁴ cfu/mL, 1×10⁴ cfu/mL and 1×10⁵ cfu/mL respectively.Four sets of 1 ml samples were prepared for testing with or without the95° C. step for PTO LM and UMO LM. Blood broth only ‘no spike’ controls(NSCs) and positive controls (broth only plus DNA polymerase (PC)) werealso prepared giving a total of 20 samples (see Table 5). Total viablecount (TVC) plates were prepared to confirm cfu/mL values (see Table 7)and negative blood broth.

To each 1 mL sample, 330 μL Reagent A was added and mixed by five tubeinversions. Samples were incubated for 15 minutes at room temperature(approximately 19° C.) and then centrifuged for 3 minutes at 7300 RCF.Following centrifugation, supernatants were decanted into a clinicalwaste receptacle and the open tubes blotted on sterile tissue paper.Each pellet was resuspended in 750 μL Reagent B by tip mixing andincubated for 5 minutes at room temperature. Next, 500 μL Reagent C wasadded to each sample and mixed by three tube inversions. Samples werecentrifuged for 3 minutes at 7300 RCF. The resulting supernatants weredecanted into a clinical waste receptacle and the open tubes blotted onsterile tissue paper. Each pellet was resuspended in 500 μL Reagent C bytip mixing, transferred to a beadmill tube containing glass beads (0.1mm and 0.5 mm glass beads), and centrifuged for 3 minutes at 7300 RCF.Following centrifugation, supernatants were transferred to waste bypipette. 50 μL LM was added to each sample and an additional 10 μL ofDNA Polymerase solution was added to the positive control samples.Samples were then placed into a Disruptor Genie and run for 6 min at2800 rpm. After bead milling, samples were transferred to a heat blockset at 37° C. and incubated for 20 minutes.

Following the 37° C. microorganism lysis (ETGA) step, the 95° C. (−)samples (samples 11-20) were progressed immediately to QPCR setup,whilst the 95° C. (+) samples (samples 1-10) were incubated at 95° C.for 5 minutes prior to QPCR setup. Both sets of samples were analysed byQPCR immediately. The same samples were analysed by QPCR again after 2hours at room temperature, and again after 24 hours, 48 hours and 72hours at stored at 4° C. This experiment was replicated three times toallow for statistical analysis of the results.

TABLE 5 Test Samples 95° C. (+) Samples 1. E. coli 1 × 10⁴ cfu/mL: PTOLM 2. S. aureus 1 × 10⁴ cfu/mL: PTO LM 3. C. albicans 1 × 10⁵ cfu/mL:PTO LM 4. PC: PTO LM 5. NSC: PTO LM 6. E. coli 1 × 10⁴ cfu/mL: UMO LM 7.S. aureus 1 × 10⁴ cfu/mL: UMO LM 8. C. albicans 1 × 10⁵ cfu/mL: UMO LM9. PC: UMO LM 10. NSC: UMO LM 95° C. (−) Samples 11. E. coli 1 × 10⁴cfu/mL: PTO LM 12. S. aureus 1 × 10⁴ cfu/mL: PTO LM 13. C. albicans 1 ×10⁵ cfu/mL: PTO LM 14. PC: PTO LM 15. NSC: PTO LM 16. E. coli 1 × 10⁴cfu/mL: UMO LM 17. S. aureus 1 × 10⁴ cfu/mL: UMO LM 18. C. albicans 1 ×10⁵ cfu/mL: UMO LM 19. PC: UMO LM 20. NSC: UMO LM PC: positive control,NSC: no spike control, UMO: unmodified oligonucleotide, PTO:phosphorothioate oligonucleotide, LM: lysis mix

TABLE 6 Materials Batch/Lot Reagent Supplier number Expiry date SheepBlood TCS Bioscience 30112000 27 Apr. 2015 (Replicate 1) Sheep Blood TCSBioscience 30210900 25 May 2015 (Replicate 2) Sheep Blood TCS Bioscience30255300 8 Jun. 2015 (Replicate 3)

TABLE 7 Microorganism TVCs Exp. 1 TVC Exp. 2 TVC Exp. 3 TVCMicroorganism (cfu total) (cfu total) (cfu total) E. coli 7,200 10,20028,200 S. aureus 4,200 23,000 23,200 C. albicans 271,000 191,000 246,000

Results and Discussion

The results for samples processed using either PTO LM or UMO LM with orwithout the 95° C. step at time 0, 2, 24, 48 and 72 hours (n=3) areshown in FIGS. 12A-E.

Removal of the 95° C. Step Improves ETGA QPCR Signal

All microorganism-spiked blood broth samples and positive controlsamples processed without the 95° C. step produced lower Ct values(stronger ETGA signal) than corresponding samples processed with the 95°C. step, for both PTO LM and UMO LM. The average ΔCt values (95° C. (+)subtract 95° C. (−)) for PTO LM samples at ‘0 hours’ were 0.98 Ct units,1.41 Ct units, 0.12 Ct units and 1.21 Ct units for E. coli, S. aureusand C. albicans and positive control respectively. The average ΔCtvalues (95° C. (+) subtract 95° C. (−)) for UMO LM samples at ‘0 hours’were 3.91 Ct units, 4.10 Ct units, 5.36 Ct units and 1.20 Ct units forE. coli, S. aureus and C. albicans and positive control respectively.The majority of NSC samples processed with PTO LM did not produce Ctvalues due to low QPCR amplification, and therefore this data is notshown in FIGS. 12A-E. However, the incidence of sufficient amplificationfor Ct values was higher for 95° C. (−) samples than 95° C. (+) samples(7/15 Ct values compared to 2/15 Ct values with no obvious trends forstorage duration; and all NSC PTO LM Ct values were between 42.0 Ctunits and 45.0 Ct units). NSC samples processed with UMO LM produced Ctvalues at ‘0 hours’ that were on average 0.42 Ct units lower without the95° C. step. This increase in QPCR signal for NSC samples processedwithout the 95° C. step is expected given the general increase in signalobserved for positive samples.

Cognitor Minus Results are More Consistent Across Different Time Pointswhen Using PTO LM Rather than UMO LM

Within the PTO LM dataset, all microorganism-spiked blood broth samplesand positive control samples produced highly consistent Ct values acrossthe 72 hour storage period, regardless of whether samples were processedwith the 95° C. step. Within the UMO LM 95° C. (+) dataset, E. coli, S.aureus and positive control samples produced fairly consistent Ct valuesacross the 72-hour storage period, whereas C. albicans and NSC samplesshowed an increase in Ct values over time (reduction in ETGA signal).This increase in Ct values for C. albicans and NSC samples may be due toa greater impact of nuclease activity on ETGA template DNA concentrationwhen the starting concentration is lower, as indicated by higher Ctvalues for these samples at ‘0 hours’. UMO LM 95° C. (−) samplesgenerally showed a decrease in Ct value (increase in ETGA QPCR signal)over time, except for C. albicans and NSC samples were Ct values werehighly consistent across the 72-hour period. This indicates that for E.coli, S. aureus and positive control samples processed without the 95°C. step (protein denaturation), continuation of the ETGA reactionresults in increased QPCR signal, out competing any nuclease activity.Whereas, in C. albicans and NSC samples, where the effect of nucleasedegradation seems to be more pronounced, the continued ETGA reaction maybe counteracting this degradation to provide more stable QPCR signalover time.

Statistical Analysis of Results

Linear modelling was performed (using R) to determine whether there arestatistically significant differences between the Ct values obtained forPTO LM and UMO LM samples processed with or without the 95° C. step atdifferent time points. For each dataset (e.g. E. coli with PTO LMdataset), ‘Ct’ was modelled against the following explanatory variablesand their interactions: ‘Log 10 cfu’, ‘Time’, and ‘95° C. step’.Non-significant (p>0.05) interactions and variables were removed fromthe model in a stepwise manner, resulting in model simplification.However, non-significant variables were not removed from the model ifany of their interactions were significant (p<0.05). Therefore, thefinal model for each dataset contained only significant interactions,significant variables, and non-significant variables that formsignificant interactions. Significance codes for each variable andinteraction are shown in Table 8 (significance codes are based onp-values for each interaction or variable at the point of modelsimplification, if removed from the model, or from the final model).

TABLE 8 Significance codes for p-values of each explanatory variable andtheir interactions using linear modelling in R Log10 Log10 Log10 cfu:cfu: Time: Log10 cfu: Dataset cfu Time 95° C. Time 95° C. 95° C. Time:95° C. E. coli PTO LM *** NS *** NS ~ NS NS E. coli UMO LM *** ** *** NSNS * NS S. aureus PTO LM *** NS * NS ** NS NS S. aureus UMO LM *** ****** NS *** ** NS C. albicans PTO LM * NS *** NS ** NS NS C. albicans UMOLM ~ NS ** NS ** NS NS PC PTO LM ~ *** NS PC ~ *** ~ UMO LM NSC UMO LMNS NS *** Significance codes: *** p < 0.001, ** p < 0.01, * p < 0.05, ~p < 0.1, NS—not significant.

The 95° C. step has a significant effect on Ct value for allmicroorganisms and positive controls with PTO LM and UMO LM. Samplestorage duration (‘Time’ and ‘Time’ interactions) has no significanteffect on Ct value for any microorganism or positive control with PTOLM; and is also non-significant for C. albicans and the positive controlwith UMO LM. However, sample storage duration is a significant variablefor E. coli and S. aureus with UMO LM: most likely due to the observedreduction in Ct value over time for samples processed without the 95° C.step in these sample sets. There is no significant effect of the 95° C.step for the NSC UMO LM dataset; but there is a significant interactionbetween sample storage duration and the 95° C. step (Time:95° C.′).Statistical analysis could not be performed on the NSC PTO LM datasetdue to missing Ct values as a result of low QPCR amplification.

SUMMARY

The data shown here demonstrate that ETGA template DNA detection isimproved when the 95° C. step is removed. Furthermore, ETGA QPCR signaldoes not deteriorate with increased sample storage duration in theabsence of the 95° C. step when samples are processed using PTO ETGAsubstrate DNA. ETGA signal is not as stable when samples are processedusing UMO ETGA substrate DNA: in the absence of the 95° C. step ETGAQPCR signal continues to increase with sample storage duration; and withthe 95° C. step ETGA QPCR signal is more likely to deteriorate as aresult of nuclease degradation without continued production of ETGAtemplate DNA. These results are consistent with the results presented inExample 3.

All of the data shown here support removal of the 95° C. step from theCognitor Minus test, and confirm the importance of using PTO ETGAsubstrate DNA in the LM. These results also verify that samplesprocessed using PTO LM can be stored for up to 72 hours at 4° C.,without being detrimental to test results. It is worth noting that theincreased sensitivity of the test upon removal of the 95° C. step mayincrease the chance of detecting background signal (blood-derived ETGAQPCR signal). However, whilst NSC PTO LM samples did not provide acomplete set of Ct values for comparison (due to low amplification), theNSC UMO LM dataset demonstrates that the increase in ETGA QPCR signalassociated with removal of the 95° C. is lower for NSCs than it is forpositive samples. Furthermore, optimisation of other factors such asblood lysis/wash performance and interpretation of QPCR results shouldeliminate the impact of this.

The present invention is not to be limited in scope by the specificembodiments described herein. Indeed, various modifications of theinvention in addition to those described herein will become apparent tothose skilled in the art from the foregoing description and accompanyingfigures. Such modifications are intended to fall within the scope of theappended claims. Moreover, all embodiments described herein areconsidered to be broadly applicable and combinable with any and allother consistent embodiments, as appropriate.

Various publications are cited herein, the disclosures of which areincorporated by reference in their entireties.

The invention claimed is:
 1. A method of detecting the presence of amicro-organism in a sample, the method comprising: (a) contacting thesample with a nuclease resistant nucleic acid molecule comprising aplurality of nuclease resistant nucleotides which is either extended bypolymerase activity or is ligated by ligase activity of themicro-organism in the sample, wherein the nuclease resistant nucleotidescomprise phosphorothioate nucleotides, (b) incubating the thus contactedsample under conditions suitable for polymerase activity or ligaseactivity; and (c) detecting in the sample a nucleic acid molecule thathas been extended by the polymerase activity or ligated by the ligaseactivity of the micro-organism as compared to a negative control,thereby indicating the presence of the micro-organism in the sample. 2.The method of claim 1 wherein the action of the polymerase activity orligase activity on the nuclease resistant nucleic acid molecule producesan extended nucleic acid molecule.
 3. The method of claim 1 wherein step(a) comprises contacting the sample with a nucleic acid molecule whichis either extended by polymerase activity or is ligated by ligaseactivity of the micro-organism in the sample together with an internalpositive control (IPC) nucleic acid molecule, wherein: (a) the IPCnucleic acid molecule is susceptible to nuclease activity and is used toidentify contaminating nuclease activity in the sample; or (b) the IPCnucleic acid molecule is nuclease resistant and comprises a plurality ofnucleotides that are resistant to nuclease activity.
 4. The method ofclaim 3 wherein a nucleic acid probe is added in step (c) which binds toa target probe sequence within the nucleic acid molecule, optionallywherein the nucleic acid probe is labelled.
 5. The method of claim 4wherein a further nucleic acid probe is added in step (c) which binds toa target probe sequence within the IPC nucleic acid molecule, optionallywherein the further nucleic acid probe is labelled.
 6. The method ofclaim 5 wherein the nucleic acid probe does not bind to the IPC nucleicacid molecule and the further nucleic acid probe does not bind to thenucleic acid molecule.
 7. The method of claim 5 wherein the nucleic acidprobe and further nucleic acid probe are differently labelled.
 8. Themethod of claim 1 wherein the nucleic acid molecule is at leastpartially double stranded and comprises uracil residues in thecomplementary strand and step (c) comprises adding Uracil DNAGlycosylase (UDG) to the sample in order to degrade the uracil residuesin the complementary strand.
 9. The method of claim 8 wherein thecomplementary strand of the nucleic acid molecule comprises amodification at the 3′ end to prevent extension.
 10. The method of claim9 wherein the modification at the 3′ end comprises incorporation of anon-extendible nucleotide.
 11. The method of claim 10 wherein thenon-extendible nucleotide is a dideoxy nucleotide triphosphate (ddNTP),optionally wherein the ddNTP is dideoxyCytidine.
 12. The method of claim1 wherein step (c) comprises a nucleic acid amplification step.
 13. Themethod of claim 1, wherein the sample contains a non-micro-organismsource of polymerase activity or ligase activity, and the methodcomprises: (a) treating the sample under high pH conditions for no morethan 5 minutes in order to inhibit the non-micro-organism source ofpolymerase activity or ligase activity, (b) contacting the sample withthe nuclease resistant nucleic acid molecule comprising a plurality ofthe nuclease resistant nucleotides which is either extended bypolymerase activity or is ligated by ligase activity of themicro-organism in the sample, (c) incubating the thus contacted sampleunder conditions suitable for polymerase activity or ligase activity;and (d) specifically determining the presence of a modified nucleic acidmolecule resulting from the action of the polymerase activity or ligaseactivity on the nuclease resistant nucleic acid molecule to indicate thepresence of the micro-organism.
 14. The method of claim 13 wherein thetreatment under high pH conditions is stopped by adding a reagent tolower the pH.
 15. The method of claim 1 wherein the sample contains anon-micro-organism source of polymerase activity or ligase activity, andthe method comprises: (a) (i) incubation of the sample with a reagentthat lyses non-microorganisms if present in the sample but does not lysemicroorganisms in the sample, (ii) separation of the lysed cell materialand the intact microorganisms if any in the sample, (iii) contacting theseparated intact microorganisms if any in the sample with a high pHreagent and incubating for no more than 5 minutes in order to inhibitthe non-micro-organism source of polymerase activity or ligase activity,(iv) adding a pH lowering reagent in order to stop the incubation athigh pH, (v) separation of the microorganisms if present in the samplefrom the pH modifying reagents, (vi) lysis of any separatedmicroorganisms, (vii) contacting the sample with the nuclease resistantnucleic acid molecule comprising the plurality of nuclease resistantnucleotides which is either extended by polymerase activity or isligated by ligase activity of the micro-organism in the sample, (viii)incubating the thus contacted sample under conditions suitable forpolymerase activity or ligase activity, and (ix) specificallydetermining the presence of the extended or ligated nucleic acidmolecule resulting from the action of the polymerase activity or ligaseactivity on the nuclease resistant nucleic acid molecule to indicate theabsence or presence of the micro-organism; or (b) (i) incubation of thesample with a reagent that lyses non-micro-organisms if present in thesample but does not lyse micro-organisms in the sample, (ii)centrifugation of the sample to form a pellet containing micro-organismsif present in the sample, (iii) removal of the supernatant from thepellet, (iv) re-suspending the pellet in a high pH reagent andincubating for no more than 8 minutes in order to inhibit thenon-micro-organism source of polymerase activity or ligase activity, (v)adding a pH lowering reagent in order to stop the incubation at high pH,(vi) a second centrifugation of the sample to form a pellet containingmicro-organisms if present in the sample, (vi) removal of thesupernatant from the pellet, (vii) lysing any micro-organisms in thepellet, (viii) contacting the sample with the nuclease resistant nucleicacid molecule comprising the plurality of nuclease resistant nucleotideswhich is either extended by polymerase activity or is ligated by ligaseactivity of the micro-organism in the sample, (ix) incubating the thuscontacted sample under conditions suitable for polymerase activity orligase activity, and (x) specifically determining the presence of theextended or ligated nucleic acid molecule resulting from the action ofthe polymerase activity or ligase activity on the nuclease resistantnucleic acid molecule to indicate the presence of the micro-organism.16. The method of claim 15 wherein steps (a)(vi) and (a)(vii) or b(vii)and b(viii) are performed together, optionally wherein the nucleic acidmolecule is added to the sample together with a lysis reagent.
 17. Themethod of claim 1 wherein the sample potentially contains anon-micro-organism source of nuclease activity and the method comprises:(i) incubation of the sample with a reagent that lysesnon-micro-organisms if present in the sample but does not lysemicro-organisms in the sample, (ii) separation of the lysed cellmaterial from the intact microorganisms if any in the sample and/orinactivation of the lysed cell material, (iii) lysing any microorganismsfollowing the separation and/or inactivation, (iv) contacting the samplewith the nuclease resistant nucleic acid molecule comprising a pluralityof the nuclease resistant nucleotides which is either extended bypolymerase activity or ligated by ligase activity of the micro-organismin the sample, (v) incubating the thus contacted sample under conditionssuitable for polymerase activity or ligase activity, and (vi)specifically determining the presence of the extended or ligated nucleicacid molecule resulting from the action of the polymerase activity orligase activity on the nuclease resistant nucleic acid molecule toindicate the presence of the micro-organism; or (a) centrifugation ofthe sample to form a pellet containing micro-organisms if present in thesample, (b) removal of the supernatant from the pellet, (c) lysing anymicro-organisms in the pellet, (d) contacting the sample with thenuclease resistant nucleic acid molecule comprising the plurality ofnuclease resistant nucleotides which is either extended by polymeraseactivity or is ligated by ligase activity of the micro-organism in thesample, (e) incubating the thus contacted sample under conditionssuitable for polymerase activity or ligase activity, and (f)specifically determining the presence of the extended or ligated nucleicacid molecule resulting from the action of the polymerase activity orligase activity on the nuclease resistant nucleic acid molecule toindicate the presence of the micro-organism.
 18. The method of claim 17wherein steps (iii) and (iv) or (c) and (d) respectively are performedtogether, optionally wherein the nuclease resistant nucleic acidmolecule comprising a plurality of nuclease resistant nucleotides isadded to the sample together with a lysis reagent.
 19. A method ofdetecting the absence of a micro-organism in a sample, the methodcomprising: (a) contacting the sample with a nuclease resistant nucleicacid molecule comprising a plurality of nuclease resistant nucleotideswhich is either extended by polymerase activity or is ligated by ligaseactivity of the micro-organism in the sample, wherein the nucleaseresistant nucleotides comprise phosphorothioate nucleotides, (b)incubating the thus contacted sample under conditions suitable forpolymerase activity or ligase activity; and (c) detecting in the samplea lack of a nucleic acid molecule that has been extended by thepolymerase activity or ligated by the ligase activity of themicro-organism as compared to a positive control, thereby indicating theabsence of the micro-organism in the sample.
 20. The method of claim 19wherein the positive control is an internal positive control (IPC). 21.The method of claim 19 wherein the nucleic acid molecule is at leastpartially double stranded and comprises uracil residues in thecomplementary strand and step (c) comprises adding Uracil DNAGlycosylase (UDG) to the sample in order to degrade the uracil residuesin the complementary strand.
 22. The method of claim 21 wherein thecomplementary strand of the nucleic acid molecule comprises amodification at the 3′ end to prevent extension.
 23. The method of claim19 wherein step (c) comprises a nucleic acid amplification step.